Source:http://linkedlifedata.com/resource/pubmed/id/19041358
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
2009-1-22
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| pubmed:abstractText |
The ideal adjuvants for hepatitis B vaccines should be capable of eliciting both strong humoral and cellular immune responses, especially Th1 cell and cytotoxic T lymphocyte (CTL) responses. However, Alum used as adjuvants in the hepatitis B vaccines currently commercialized offers limitation in inducing cell-mediated response. Therefore, a less hemolytic saponin platycodin D (PD) from the root of Platycodon grandiflorum has been explored for its potential as an alternative adjuvant. In order to compare the adjuvant activity with Alum, antigen-specific cellular and humoral immune responses were evaluated following immunization with a formulation containing hepatitis B surface antigen (HBsAg) adjuvanted with PD and Alum in mice. The Con A-, LPS-, and HBsAg-induced splenocyte proliferation and the serum HBsAg-specific IgG, IgG1, IgG2a, and IgG2b antibody titers in the HBsAg-immunized mice were significantly enhanced by PD (P<0.05, P<0.01 or P<0.001). PD also significantly promoted the production of Th1 (IL-2 and IFN-gamma) and Th2 (IL-10) cytokines and up-regulated the mRNA expression of Th1 cytokines (IL-2 and IFN-gamma) in splenocytes from the mice immunized with HBsAg (P<0.001). Besides, PD remarkably increased the killing activities of natural killer (NK) cells and CTLs from splenocytes in the HBsAg-immunized mice (P<0.001), which may have important implications for vaccination against hepatitis B virus. The results indicated that PD has strong potential to increase both cellular and humoral immune responses and elicit a balanced Th1/Th2 response against HBsAg, and that PD may be the candidates as adjuvants for use in prophylactic and therapeutic hepatitis B vaccine.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adjuvants, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Alum Compounds,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis B Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis B Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatitis B Vaccines,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Saponins,
http://linkedlifedata.com/resource/pubmed/chemical/Triterpenes,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, Synthetic,
http://linkedlifedata.com/resource/pubmed/chemical/aluminum sulfate,
http://linkedlifedata.com/resource/pubmed/chemical/platycodin D
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0264-410X
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
29
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| pubmed:volume |
27
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
757-64
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| pubmed:meshHeading |
pubmed-meshheading:19041358-Adjuvants, Immunologic,
pubmed-meshheading:19041358-Alum Compounds,
pubmed-meshheading:19041358-Animals,
pubmed-meshheading:19041358-Cell Proliferation,
pubmed-meshheading:19041358-Cytokines,
pubmed-meshheading:19041358-Female,
pubmed-meshheading:19041358-Hepatitis B,
pubmed-meshheading:19041358-Hepatitis B Antibodies,
pubmed-meshheading:19041358-Hepatitis B Antigens,
pubmed-meshheading:19041358-Hepatitis B Vaccines,
pubmed-meshheading:19041358-Immunoglobulin G,
pubmed-meshheading:19041358-Killer Cells, Natural,
pubmed-meshheading:19041358-Mice,
pubmed-meshheading:19041358-Platycodon,
pubmed-meshheading:19041358-Saponins,
pubmed-meshheading:19041358-Spleen,
pubmed-meshheading:19041358-T-Lymphocytes,
pubmed-meshheading:19041358-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:19041358-Triterpenes,
pubmed-meshheading:19041358-Vaccines, Synthetic
|
| pubmed:year |
2009
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| pubmed:articleTitle |
Platycodin D is a potent adjuvant of specific cellular and humoral immune responses against recombinant hepatitis B antigen.
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| pubmed:affiliation |
Key Laboratory of Animal Epidemic Etiology & Immunological Prevention of Ministry of Agriculture, College of Animal Sciences, Zhejiang University, Kaixuan Road 268, Hangzhou, Zheijiang 310029, China.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|