Linked Life Data

1904001

Source:http://linkedlifedata.com/resource/pubmed/id/1904001

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1991-7-10
pubmed:abstractText
The hyperglycemia-induced in situ metabolism and blood flow changes produced in s.c. implanted murine radiation-induced fibrosarcoma-1 tumors, grown on the flanks of female C3H/HeJ mice, were examined with 31P and 2H nuclear magnetic resonance. Initial experiments verified a hyperglycemic tumor acidification similar to that reported earlier with a different substrain of mice, C3H/AnF (J.L. Evelhoch et al., Proc. Natl. Acad. Sci. USA, 81: 6496-6500, 1984). Changes in the tumor pH, phosphorus metabolites, and blood flow were then compared after administration of saline, glucose, or mannitol (a nonmetabolizable glucose analogue) using a mole-equivalent dose of the sugars (i.e., 0.8 mmol/20g mouse). Neither saline (n = 8) nor mannitol (n = 6) administration had any marked effect upon tumor pH, whereas glucose administration produced a mean maximum tumor pH reduction of 0.74 +/- 0.09 (SE; n = 9) during the 2.5 h post-glucose injection. No significant changes in high energy phosphate concentrations were observed during the same period after saline injection. After glucose injection, the [phosphocreatine] gradually decreased by 64% (P = 0.0001). After the initial 1 h post-glucose injection, the [inorganic phosphate] increased by 58% (P = 0.0001), and the [nucleoside triphosphates] decreased by 29% (P = 0.0001) during the following 1.5 h. After mannitol injection, while there was no change in [inorganic phosphate] over time (P = 0.37), the [phosphocreatine] decreased by 33% (P = 0.0001) and the [nucleoside triphosphates] decreased by 21% (P = 0.0015) within 20 min, then both the [phosphocreatine] and [nucleoside triphosphates] remained at constant levels during the following 2 h. In parallel experiments, the volumetric rate of tumor blood flow and perfusion was measured by 2H nuclear magnetic resonance monitoring of 2H2O washout kinetics (S-G. Kim and J. J. H. Ackerman, Cancer Res., 48: 3449-3453, 1988); tumor blood flow decreased by 80% (P = 0.0001, n = 11), 60% (P = 0.0031, n = 4), and 20% (P = 0.058, n = 10) at 2 h after glucose, mannitol, or saline injections, respectively. These results suggest that anaerobic glycolysis is a requirement for hyperglycemic tumor acidification. However, the decrease in tumor blood flow accompanying hyperglycemic acidification suggests that flow reduction also may be a contributing or a required cofactor for acidification via inhibition of lactic acid egress.(ABSTRACT TRUNCATED AT 400 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
51
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3108-18
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:1904001-Animals, pubmed-meshheading:1904001-Blood Glucose, pubmed-meshheading:1904001-Carbon Dioxide, pubmed-meshheading:1904001-Deuterium, pubmed-meshheading:1904001-Female, pubmed-meshheading:1904001-Fibrosarcoma, pubmed-meshheading:1904001-Glucose, pubmed-meshheading:1904001-Hematocrit, pubmed-meshheading:1904001-Hydrogen-Ion Concentration, pubmed-meshheading:1904001-Kinetics, pubmed-meshheading:1904001-Magnetic Resonance Spectroscopy, pubmed-meshheading:1904001-Mannitol, pubmed-meshheading:1904001-Mice, pubmed-meshheading:1904001-Mice, Inbred C3H, pubmed-meshheading:1904001-Neoplasms, Radiation-Induced, pubmed-meshheading:1904001-Oxygen, pubmed-meshheading:1904001-Partial Pressure, pubmed-meshheading:1904001-Phosphocreatine, pubmed-meshheading:1904001-Phosphorus, pubmed-meshheading:1904001-Regional Blood Flow, pubmed-meshheading:1904001-Ribonucleotides, pubmed-meshheading:1904001-Sarcoma, Experimental
pubmed:year
1991
pubmed:articleTitle
Modulation of murine radiation-induced fibrosarcoma-1 tumor metabolism and blood flow in situ via glucose and mannitol administration monitored by 31P and 2H nuclear magnetic resonance spectroscopy.
pubmed:affiliation
Department of Chemistry, Washington University, St. Louis, Missouri 63130.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.