Linked Life Data

19038823

Source:http://linkedlifedata.com/resource/pubmed/id/19038823

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2008-11-28
pubmed:abstractText
The aim of this study was to determine whether chicken sperm express avian beta-defensin 3 (AvBD-3), an antimicrobial peptide, and retain it during the residence in the male and female reproductive tract. Immunocytochemistry to identify AvBD-3 was performed in the testis, epididymis, ductus deferens, ejaculated sperm, and utero-vaginal junction of the oviduct of inseminated hens. Reverse transcription-PCR analysis was also performed to confirm the synthesis of AvBD-3 by testicular cells and ejaculated sperm. The immunoreaction products for AvBD-3 were identified in the elongated spermatids attached to the seminiferous epithelium and in the sperm in the lumen of seminiferous tubules. Immunoreaction products were found in the sperm in the epididymal ducts and ductus deferens. In the ejaculated sperm, immunoreaction products were distributed in the midpiece and cephalic region of the tail of sperm, but not in the head. The AvBD-3 immunoreaction products were also identified in the sperm stored in the sperm storage tubules in the utero-vaginal junction. Reverse transcription-PCR analysis showed the expression of AvBD-3 mRNA in the testicular tissue, but not in the ejaculated sperm. These results suggest that AvBD-3 is synthesized by late stage of spermatids in the testis, and this molecule is retained by the sperm during the passage of male reproductive tract, and even in the sperm storage tubules of oviduct. The sperm AvBD-3 is assumed to play roles in protecting the sperm from the microbial infection and enable to survive in the male and female reproductive tracts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0032-5791
pubmed:author
pubmed:issnType
Print
pubmed:volume
87
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2653-9
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Expression of avian beta-defensin 3, an antimicrobial peptide, by sperm in the male reproductive organs and oviduct in chickens: an immunohistochemical study.
pubmed:affiliation
Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't