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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2008-12-29
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pubmed:abstractText |
Mesenchymal stem cells (MSCs) have received special attention for cardiomyoplasty because several studies have shown that they differentiate into cardiomyocytes both in vitro and in vivo. Nitric oxide (NO) is a free radical signaling molecule that regulates several differentiation processes including cardiomyogenesis. Here, we report an investigation of the effects of two NO agents (SNAP and DEA/NO), able to activate both cGMP-dependent and -independent pathways, on the cardiomyogenic potential of bone marrow-derived mesenchymal stem cells (BM-MSCs) and adipose tissue-derived stem cells (ADSCs). The cells were isolated, cultured and treated with NO agents. Cardiac- and muscle-specific gene expression was analyzed by indirect immunofluorescence, flow cytometry, RT-PCR and real-time PCR. We found that untreated (control) ADSCs and BM-MSCs expressed some muscle markers and NO-derived intermediates induce an increased expression of some cardiac function genes in BM-MSCs and ADSCs. Moreover, NO agents considerably increased the pro-angiogenic potential mostly of BM-MSCs as determined by VEGF mRNA levels.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1,1-diethyl-2-hydroxy-2-nitrosohydra...,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Connexin 43,
http://linkedlifedata.com/resource/pubmed/chemical/Genetic Markers,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrazines,
http://linkedlifedata.com/resource/pubmed/chemical/Muscle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Donors,
http://linkedlifedata.com/resource/pubmed/chemical/Penicillamine,
http://linkedlifedata.com/resource/pubmed/chemical/S-nitro-N-acetylpenicillamine,
http://linkedlifedata.com/resource/pubmed/chemical/Vascular Endothelial Growth Factor A
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1090-2104
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pubmed:author |
pubmed-author:AguiarAlessandra MAM,
pubmed-author:AitaCarlos MCM,
pubmed-author:BarchikiFabianeF,
pubmed-author:BrofmanPaulo SPS,
pubmed-author:CorreaAlejandroA,
pubmed-author:DallagiovannaBrunoB,
pubmed-author:GoldenbergSamuelS,
pubmed-author:HansenPaulaP,
pubmed-author:KuligovskiCriscieleC,
pubmed-author:MoutinhoJosé AJA,
pubmed-author:NakaoLia SLS,
pubmed-author:OlandoskiMárciaM,
pubmed-author:RebelattoCarmen KCK,
pubmed-author:SenegagliaAlexandra CAC
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pubmed:issnType |
Electronic
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pubmed:day |
16
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pubmed:volume |
378
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
456-61
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pubmed:meshHeading |
pubmed-meshheading:19032948-Adult,
pubmed-meshheading:19032948-Adult Stem Cells,
pubmed-meshheading:19032948-Aged,
pubmed-meshheading:19032948-Antigens, CD,
pubmed-meshheading:19032948-Cardiomyoplasty,
pubmed-meshheading:19032948-Cell Differentiation,
pubmed-meshheading:19032948-Cells, Cultured,
pubmed-meshheading:19032948-Connexin 43,
pubmed-meshheading:19032948-Gene Expression,
pubmed-meshheading:19032948-Genetic Markers,
pubmed-meshheading:19032948-Heart,
pubmed-meshheading:19032948-Humans,
pubmed-meshheading:19032948-Hydrazines,
pubmed-meshheading:19032948-Mesenchymal Stem Cells,
pubmed-meshheading:19032948-Middle Aged,
pubmed-meshheading:19032948-Multipotent Stem Cells,
pubmed-meshheading:19032948-Muscle Proteins,
pubmed-meshheading:19032948-Myocytes, Cardiac,
pubmed-meshheading:19032948-Nitric Oxide,
pubmed-meshheading:19032948-Nitric Oxide Donors,
pubmed-meshheading:19032948-Penicillamine,
pubmed-meshheading:19032948-Vascular Endothelial Growth Factor A
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pubmed:year |
2009
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pubmed:articleTitle |
Expression of cardiac function genes in adult stem cells is increased by treatment with nitric oxide agents.
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pubmed:affiliation |
Núcleo de Cardiomioplastia Celular, Pontifícia Universidade Católica do Paraná, Rua Imaculada Conceição 1155, Curitiba 80215-901, Brazil.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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