rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
2008-12-16
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pubmed:abstractText |
Identification of reliable markers of chemo- and radiosensitivity and the key molecules that enhance the susceptibility of squamous esophageal cancer cells to anticancer treatments would be highly desirable. To test whether regenerating gene (REG) I expression enhances chemo- and radiosensitivity in esophageal squamous cell carcinoma cells, we used MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assays to compare the chemo- and radiosensitivities of untransfected TE-5 and TE-9 cells with those of cells stably transfected with REG Ialpha and Ibeta. We then used flow cytometry to determine whether REG I expression alters cell cycle progression. No REG I mRNA or protein were detected in untransfected TE-5 and TE-9 cells. Transfection with REG Ialpha and Ibeta led to strong expression of both REG I mRNA and protein in TE-5 and TE-9 cells, which in turn led to significant increases in both chemo- and radiosensitivity. Cell cycle progression was unaffected by REG I expression. REG I thus appears to enhance the chemo- and radiosensitivity of squamous esophageal cancer cells, which suggests that it may be a useful target for improved and more individualized treatments for patients with esophageal squamous cell carcinoma.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antimetabolites, Antineoplastic,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorouracil,
http://linkedlifedata.com/resource/pubmed/chemical/Formazans,
http://linkedlifedata.com/resource/pubmed/chemical/Lithostathine,
http://linkedlifedata.com/resource/pubmed/chemical/MTT formazan,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/REG1A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrazolium Salts
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1349-7006
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pubmed:author |
pubmed-author:BensoLL,
pubmed-author:HayashiKaoriK,
pubmed-author:Itaya-HironakaAsakoA,
pubmed-author:KoizumiYukioY,
pubmed-author:KoyotaSouichiS,
pubmed-author:MaruyamaKiyotomiK,
pubmed-author:MinamiyaYoshihiroY,
pubmed-author:MotoyamaSatoruS,
pubmed-author:OgawaJun-IchiJ,
pubmed-author:SaitoHajimeH,
pubmed-author:Sakuramoto-TsuchidaSumiyoS,
pubmed-author:SugiyamaToshihiroT,
pubmed-author:TakasawaShinS
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pubmed:issnType |
Electronic
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pubmed:volume |
99
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2491-5
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pubmed:meshHeading |
pubmed-meshheading:19032369-Antimetabolites, Antineoplastic,
pubmed-meshheading:19032369-Carcinoma, Squamous Cell,
pubmed-meshheading:19032369-Cell Line, Tumor,
pubmed-meshheading:19032369-Cell Proliferation,
pubmed-meshheading:19032369-Dose-Response Relationship, Radiation,
pubmed-meshheading:19032369-Esophageal Neoplasms,
pubmed-meshheading:19032369-Fluorouracil,
pubmed-meshheading:19032369-Formazans,
pubmed-meshheading:19032369-Humans,
pubmed-meshheading:19032369-Lithostathine,
pubmed-meshheading:19032369-Proteins,
pubmed-meshheading:19032369-RNA, Messenger,
pubmed-meshheading:19032369-Radiation Tolerance,
pubmed-meshheading:19032369-Tetrazolium Salts,
pubmed-meshheading:19032369-Transfection
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pubmed:year |
2008
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pubmed:articleTitle |
REG I enhances chemo- and radiosensitivity in squamous cell esophageal cancer cells.
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pubmed:affiliation |
Department of Biochemistry, Akita University School of Medicine, 1-1-1 Hondo, Akita 010-8543, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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