| pubmed-article:19029770 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19029770 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:19029770 | lifeskim:mentions | umls-concept:C0997643 | lld:lifeskim |
| pubmed-article:19029770 | lifeskim:mentions | umls-concept:C0022949 | lld:lifeskim |
| pubmed-article:19029770 | lifeskim:mentions | umls-concept:C0086045 | lld:lifeskim |
| pubmed-article:19029770 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:19029770 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:19029770 | pubmed:dateCreated | 2008-11-25 | lld:pubmed |
| pubmed-article:19029770 | pubmed:abstractText | We investigated the production of galactooligosaccharides (GOS) from lactose by alginate-immobilized cells of Sporobolomyces singularis YIT 10047 (IM-SS). beta-Galactosidase activity was stable at 30 to 50 degrees C but decreased dramatically between 50 and 60 degrees C and disappeared at 70 degrees C in acetate buffer. The enzyme activity remaining was no more than 20% of that of unheated samples after incubation in distilled water at 55 degrees C, whereas its value was about 60% at the same temperature under buffered condition. However, activity was maintained more than 80% with 10% to 50% (w/w) lactose after incubation at 55 degrees C without buffer. In a single-batch reaction, GOS yield was 41.0% with free cells and 40.4% with IM-SS. We attempted a repeated-batch reaction using IM-SS with 600 g L(-1)lactose. IM-SS produced GOS stably for 20 batches (22 h/batch, 440 h in total) at 55 degrees C and pH 5.0 or 6.0. IM-SS produced GOS at 242 g L(-1), at a rate of 8.72 g L(-1) h(-1). Both GOS yield and production rate were higher than those in published experiments on GOS production using immobilized biocatalysts. The repeated-batch reaction with IM-SS would be an ideal system for GOS production because of its stability and high productivity. | lld:pubmed |
| pubmed-article:19029770 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19029770 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19029770 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19029770 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19029770 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19029770 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19029770 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19029770 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19029770 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19029770 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19029770 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19029770 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19029770 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19029770 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:19029770 | pubmed:issn | 0022-1260 | lld:pubmed |
| pubmed-article:19029770 | pubmed:author | pubmed-author:SakaiTakafumi... | lld:pubmed |
| pubmed-article:19029770 | pubmed:author | pubmed-author:HayakawaKazuh... | lld:pubmed |
| pubmed-article:19029770 | pubmed:author | pubmed-author:TsujiHirokazu... | lld:pubmed |
| pubmed-article:19029770 | pubmed:author | pubmed-author:MatsumotoKeis... | lld:pubmed |
| pubmed-article:19029770 | pubmed:author | pubmed-author:ShibataShinya... | lld:pubmed |
| pubmed-article:19029770 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:19029770 | pubmed:volume | 54 | lld:pubmed |
| pubmed-article:19029770 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19029770 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19029770 | pubmed:pagination | 285-93 | lld:pubmed |
| pubmed-article:19029770 | pubmed:meshHeading | pubmed-meshheading:19029770... | lld:pubmed |
| pubmed-article:19029770 | pubmed:meshHeading | pubmed-meshheading:19029770... | lld:pubmed |
| pubmed-article:19029770 | pubmed:meshHeading | pubmed-meshheading:19029770... | lld:pubmed |
| pubmed-article:19029770 | pubmed:meshHeading | pubmed-meshheading:19029770... | lld:pubmed |
| pubmed-article:19029770 | pubmed:meshHeading | pubmed-meshheading:19029770... | lld:pubmed |
| pubmed-article:19029770 | pubmed:meshHeading | pubmed-meshheading:19029770... | lld:pubmed |
| pubmed-article:19029770 | pubmed:meshHeading | pubmed-meshheading:19029770... | lld:pubmed |
| pubmed-article:19029770 | pubmed:meshHeading | pubmed-meshheading:19029770... | lld:pubmed |
| pubmed-article:19029770 | pubmed:meshHeading | pubmed-meshheading:19029770... | lld:pubmed |
| pubmed-article:19029770 | pubmed:meshHeading | pubmed-meshheading:19029770... | lld:pubmed |
| pubmed-article:19029770 | pubmed:meshHeading | pubmed-meshheading:19029770... | lld:pubmed |
| pubmed-article:19029770 | pubmed:meshHeading | pubmed-meshheading:19029770... | lld:pubmed |
| pubmed-article:19029770 | pubmed:year | 2008 | lld:pubmed |
| pubmed-article:19029770 | pubmed:articleTitle | Repeated-batch production of galactooligosaccharides from lactose at high concentration by using alginate-immobilized cells of Sporobolomyces singularis YIT 10047. | lld:pubmed |
| pubmed-article:19029770 | pubmed:affiliation | Yakult Central Institute for Microbiological Research, Kunitachi, Tokyo, Japan. takafumi.sakai@yher.be | lld:pubmed |
| pubmed-article:19029770 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19029770 | pubmed:publicationType | Evaluation Studies | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:19029770 | lld:pubmed |
