Linked Life Data

19029770

Source:http://linkedlifedata.com/resource/pubmed/id/19029770

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2008-11-25
pubmed:abstractText
We investigated the production of galactooligosaccharides (GOS) from lactose by alginate-immobilized cells of Sporobolomyces singularis YIT 10047 (IM-SS). beta-Galactosidase activity was stable at 30 to 50 degrees C but decreased dramatically between 50 and 60 degrees C and disappeared at 70 degrees C in acetate buffer. The enzyme activity remaining was no more than 20% of that of unheated samples after incubation in distilled water at 55 degrees C, whereas its value was about 60% at the same temperature under buffered condition. However, activity was maintained more than 80% with 10% to 50% (w/w) lactose after incubation at 55 degrees C without buffer. In a single-batch reaction, GOS yield was 41.0% with free cells and 40.4% with IM-SS. We attempted a repeated-batch reaction using IM-SS with 600 g L(-1)lactose. IM-SS produced GOS stably for 20 batches (22 h/batch, 440 h in total) at 55 degrees C and pH 5.0 or 6.0. IM-SS produced GOS at 242 g L(-1), at a rate of 8.72 g L(-1) h(-1). Both GOS yield and production rate were higher than those in published experiments on GOS production using immobilized biocatalysts. The repeated-batch reaction with IM-SS would be an ideal system for GOS production because of its stability and high productivity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-1260
pubmed:author
pubmed:issnType
Print
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
285-93
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Repeated-batch production of galactooligosaccharides from lactose at high concentration by using alginate-immobilized cells of Sporobolomyces singularis YIT 10047.
pubmed:affiliation
Yakult Central Institute for Microbiological Research, Kunitachi, Tokyo, Japan. takafumi.sakai@yher.be
pubmed:publicationType
Journal Article, Evaluation Studies