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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1991-6-10
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pubmed:abstractText |
A differential kinetic study of 13CO2 enrichment of breath after the intake of specific 13C-labelled substrates and co-administration of a drug allows the drug's ability for enzyme induction to be evaluated in vivo. A method and a gas chromatograph-isotope ratio mass spectrometer device for on-line measurements of 13CO2 enrichment in the breath of small animals are described. This system allows on-line breath sample collection from a metabolic cage, purification by gas chromatography, determination of CO2 by thermal conductivity detection and measurement of 13CO2 enrichment by isotope ratio mass spectrometry. Two protocols for phenobarbital-inducible P450 and 3-methylcholanthrene-inducible P1-450 isoenzymes are described.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0021-9673
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
562
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
341-50
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1902840-Animals,
pubmed-meshheading:1902840-Breath Tests,
pubmed-meshheading:1902840-Caffeine,
pubmed-meshheading:1902840-Carbon Dioxide,
pubmed-meshheading:1902840-Carbon Isotopes,
pubmed-meshheading:1902840-Cytochrome P-450 Enzyme System,
pubmed-meshheading:1902840-Enzyme Induction,
pubmed-meshheading:1902840-Gas Chromatography-Mass Spectrometry,
pubmed-meshheading:1902840-Kinetics,
pubmed-meshheading:1902840-Male,
pubmed-meshheading:1902840-Methylcholanthrene,
pubmed-meshheading:1902840-Phenobarbital,
pubmed-meshheading:1902840-Rats
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pubmed:year |
1991
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pubmed:articleTitle |
On-line measurements of 13C enrichments in rat breath. Non-invasive method for in vivo study of drug enzymatic induction.
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pubmed:affiliation |
L.E.A.C.M., Laboratoire d'Etudes Analytiques et Cinétiques du Médicament, Institut des Sciences Pharmaceutiques et Biologiques de Lyon, France.
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pubmed:publicationType |
Journal Article
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