Linked Life Data

19028300

Source:http://linkedlifedata.com/resource/pubmed/id/19028300

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-11-25
pubmed:abstractText
We have previously shown a relationship between the virulence level of Listeria monocytogenes strains and their detection on PALCAM medium. To account for the fact that only 40% of low-virulence field strains of L. monocytogenes were detected on PALCAM medium compared to 92% on ALOA medium, the detection of virulent and low-virulence strains on decomposed selective ALOA and PALCAM media was compared. This showed that better detection of the strains was not explained by the growth factors added to the ALOA medium. On the other hand, the presence of acriflavine in the PALCAM medium partly explained the delay in detection of the low-virulence strains, while the presence of ceftazidime was related to growth inhibition. However, the effect of these two components was modified when they were combined in the PALCAM medium. As some of these low-virulence strains had an inactive PrfA (the transcriptional activator of the main virulence genes of L. monocytogenes), its role in the poor detection of these low-virulence strains was investigated. However, complementing these strains with the wild-type prfA gene or deleting the prfA gene from a virulent strain suggested that this poor detection was unrelated to PrfA, but was related to their higher susceptibility to the antimicrobial components in the selective media.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1095-9998
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
21-6
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Poor detection of low-virulence field strains of L. monocytogenes is related to selective agents in selective media and is unrelated to PrfA.
pubmed:affiliation
INRA, UR1282 Infectiologie Animale et Santé Publique, F-37380 Nouzilly, France. sylvie.roche@tours.inra.fr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't