Linked Life Data

19027855

Source:http://linkedlifedata.com/resource/pubmed/id/19027855

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2009-2-16
pubmed:abstractText
Sebocytes are sebum-producing cells that form the sebaceous glands. We investigated the role of sebocytes as target cells for vitamin D metabolites and the existence of an enzymatic machinery for the local synthesis and metabolism of 1,25-dihydroxyvitamin D(3) [1,25(OH)(2)D(3), calcitriol], the biologically active vitamin D metabolite, in these cell types. Expression of vitamin D receptor (VDR), vitamin D-25-hydroxylase (25 OHase), 25-hydroxyvitamin D-1alpha-hydroxylase (1 alphaOHase), and 1,25-dihydroxyvitamin D-24-hydroxylase (24 OHase) was detected in SZ95 sebocytes in vitro using real time quantitative polymerase chain reaction. Splice variants of 1alphaOHase were identified by nested touchdown polymerase chain reaction. We demonstrated that incubation of SZ95 sebocytes with 1,25(OH)(2)D(3) resulted in a cell culture condition-, time-, and dose-dependent modulation of cell proliferation, cell cycle regulation, lipid content and interleukin-6/interleukin-8 secretion in vitro. RNA expression of VDR and 24 OHase was upregulated along with vitamin D analogue treatment. Although several other splice variants of 1alphaOHase were detected, our findings indicate that the full length product represents the major 1 alphaOHase gene product in SZ95 cells. In conclusion, SZ95 sebocytes express VDR and the enzymatic machinery to synthesize and metabolize biologically active vitamin D analogues. Sebocytes represent target cells for biologically active metabolites. Our findings indicate that the vitamin D endocrine system is of high importance for sebocyte function and physiology. We conclude that sebaceous glands represent potential targets for therapy with vitamin D analogues or for pharmacological modulation of 1,25(OH)(2)D(3) synthesis/metabolism.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0960-0760
pubmed:author
pubmed:issnType
Print
pubmed:volume
113
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
9-16
pubmed:meshHeading
pubmed-meshheading:19027855-25-Hydroxyvitamin D3 1-alpha-Hydroxylase, pubmed-meshheading:19027855-Alternative Splicing, pubmed-meshheading:19027855-Blotting, Western, pubmed-meshheading:19027855-Calcifediol, pubmed-meshheading:19027855-Calcitriol, pubmed-meshheading:19027855-Cell Proliferation, pubmed-meshheading:19027855-Cells, Cultured, pubmed-meshheading:19027855-Dose-Response Relationship, Drug, pubmed-meshheading:19027855-Endocrine System, pubmed-meshheading:19027855-G1 Phase, pubmed-meshheading:19027855-Humans, pubmed-meshheading:19027855-Immunohistochemistry, pubmed-meshheading:19027855-Interleukin-6, pubmed-meshheading:19027855-Interleukin-8, pubmed-meshheading:19027855-Lipid Metabolism, pubmed-meshheading:19027855-Receptors, Calcitriol, pubmed-meshheading:19027855-Sebaceous Glands, pubmed-meshheading:19027855-Vitamin D
pubmed:year
2009
pubmed:articleTitle
Characterization of the vitamin D endocrine system in human sebocytes in vitro.
pubmed:affiliation
Department of Dermatology, The Saarland University Hospital, 66421 Homburg/Saar, Germany.
pubmed:publicationType
Journal Article