Linked Life Data

19027756

Source:http://linkedlifedata.com/resource/pubmed/id/19027756

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2009-2-2
pubmed:abstractText
Life expectancy in central-Eastern European countries is more than 10 years lower compared with Northern or Western countries which could be the result of complex factors including genetics, nutrition and life style. We conducted a molecular epidemiological study with the aim of investigating links between DNA instability, genetic polymorphisms in nucleotide excision repair genes and ageing. Two groups-151 young people (78 women and 73 men) aged 20-25, and 140 elderly subjects (101 women and 39 men), aged 65-70 have been investigated. Results show elevated levels of micronuclei and chromosome aberrations in elderly compared with young groups (P<0.001); women had more micronuclei than men (P<0.001). Micronucleus frequencies were influenced by age (P<0.001). In the group of elderly people those who were homozygous with C/C or A/A in XPC IVS11 had more aberrant cells compared with C/A heterozygotes (P=0.04). When the dependent variable was break per cell, elderly people A/A homozygous in XPC IVS11 had more breaks per cell compared with C/A heterozygous or C/C homozygous subjects (P=0.03). Significantly the most chromatid breaks were found in elderly people both Lys/Lys homozygous in the XPD Lys751Gln genotype and C/C or A/A homozygous in the XPC IVS11 genotype (P<0.05). A General Linear Model analysis shows a statistically significant effect of interactions between age, sex and genotype XPC IVS11 (P=0.001) and age, sex and genotype XPCin9 (P=0.007) on number of chromatid breaks. When we divided people into two subgroups (without mutant allele and with one or two mutant alleles) we found a significantly higher number of chromosome exchanges in people with one or two variant polymorphism XPCin9 (P=0.04), XPC IVS11 (P=0.004) or XPCex15 (P=0.001). Level of cells with micronuclei was influenced by polymorphisms XPD Lys751Gln (P=0.03). However, we did not find any relationship between XPA polymorphism and studied cytogenetic biomarkers.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0027-5107
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
661
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
35-40
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:19027756-Adult, pubmed-meshheading:19027756-Aged, pubmed-meshheading:19027756-Aging, pubmed-meshheading:19027756-Base Sequence, pubmed-meshheading:19027756-Chromosome Aberrations, pubmed-meshheading:19027756-DNA Primers, pubmed-meshheading:19027756-DNA Repair, pubmed-meshheading:19027756-DNA-Binding Proteins, pubmed-meshheading:19027756-Female, pubmed-meshheading:19027756-Genetic Markers, pubmed-meshheading:19027756-Genotype, pubmed-meshheading:19027756-Humans, pubmed-meshheading:19027756-Male, pubmed-meshheading:19027756-Micronuclei, Chromosome-Defective, pubmed-meshheading:19027756-Micronucleus Tests, pubmed-meshheading:19027756-Models, Genetic, pubmed-meshheading:19027756-Polymorphism, Single Nucleotide, pubmed-meshheading:19027756-Xeroderma Pigmentosum Group D Protein, pubmed-meshheading:19027756-Young Adult
pubmed:year
2009
pubmed:articleTitle
Micronuclei and chromosomal aberrations, important markers of ageing: possible association with XPC and XPD polymorphisms.
pubmed:affiliation
Research Base of the Slovak Medical University, Limbová 14, 833 01 Bratislava, Slovak Republic. alena.kazimirova@szu.sk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't