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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2008-12-8
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pubmed:abstractText |
Obesity is associated with a low-grade inflammation in adipose tissue resulting from increased production of pro-inflammatory cytokines and which can subsequently contribute to the development of insulin resistance. However, the mechanisms underlying the transcriptional regulation of pro-inflammatory genes are still unclear. Here we show that tumor necrosis factor (TNF)-alpha treatment attenuated Akt-dependent phosphorylation of Foxo1 and enhanced transcriptional activity of Foxo1. We found that Foxo1 increased the expression of CCAAT/enhancer binding protein (C/EBPbeta, a positive regulator of monocyte chemoattractant protein (MCP)-1 and interleukin (IL)-6 genes, through directly binding to its promoter. Furthermore, knockdown of Foxo1 as well as C/EBPbeta inhibits TNF-alpha-induced expression of MCP-1 and IL-6 in 3T3-L1 adipocytes. These findings suggest that activation of Foxo1 triggered by TNF-alpha up-regulates the expression of C/EBPbeta in 3T3-L1 adipocytes, thereby leading to an increased production of pro-inflammatory cytokines, MCP-1 and IL-6.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1090-2104
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
9
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pubmed:volume |
378
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
290-5
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:19026986-3T3-L1 Cells,
pubmed-meshheading:19026986-Adipocytes,
pubmed-meshheading:19026986-Animals,
pubmed-meshheading:19026986-CCAAT-Enhancer-Binding Protein-beta,
pubmed-meshheading:19026986-Chemokine CCL2,
pubmed-meshheading:19026986-Chromatin Immunoprecipitation,
pubmed-meshheading:19026986-Forkhead Transcription Factors,
pubmed-meshheading:19026986-Gene Expression Regulation,
pubmed-meshheading:19026986-Gene Knockdown Techniques,
pubmed-meshheading:19026986-Humans,
pubmed-meshheading:19026986-Inflammation,
pubmed-meshheading:19026986-Interleukin-6,
pubmed-meshheading:19026986-Mice,
pubmed-meshheading:19026986-Phosphorylation,
pubmed-meshheading:19026986-Promoter Regions, Genetic,
pubmed-meshheading:19026986-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:19026986-Transcription, Genetic,
pubmed-meshheading:19026986-Tumor Necrosis Factor-alpha
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pubmed:year |
2009
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pubmed:articleTitle |
Foxo1 increases pro-inflammatory gene expression by inducing C/EBPbeta in TNF-alpha-treated adipocytes.
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pubmed:affiliation |
Center for Tsukuba Advanced Research Alliance, Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba 305-8577, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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