Source:http://linkedlifedata.com/resource/pubmed/id/19022954
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
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| pubmed:dateCreated |
2008-11-21
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| pubmed:abstractText |
Matrix gamma-carboxyglutamic acid protein (MGP), a vitamin K-dependent protein, is involved in regulation of tissue calcification. We previously reported that 9-cis retinoic acid (RA) mitigates 1alpha,25-dihydroxycholecalciferol [1,25(OH)(2)D3]-induced renal calcification in a 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-induced lung cancer A/J male mouse model. This raised the question if the mechanism(s) underlying this calcification involves vitamin K. We assessed expression and vitamin K dependent gamma-carboxylation of MGP and vitamin K concentrations [phylloquinone (PK), as well as its conversion product, menaquinone-4 (MK-4)] in tissues obtained from NNK-injected A/J male mice fed 1,25(OH)(2)D3 (2.5 microg/kg diet; D group) +/- RA (15 mg/kg diet) for 20 wk. Renal calcification was only observed in the D group (2/10; 20% of the group). Renal MGP mRNA and uncarboxylated MGP (ucMGP) increased in response to D (P < 0.05) but not in response to RA or RA + D. In contrast, gamma-carboxylated MGP increased to 2.2-fold of the control in response to D+RA (P < 0.05) but not in response to RA or D alone. Although all diets contained equal amounts of PK, the kidney MK-4 concentration was higher in the D group (P < 0.05) and lower in the RA group (P < 0.05) compared with the RA+D or control groups. Renal PK concentrations were lower in the RA and RA+D groups than in the control and D groups (P < 0.05). These data suggest that 9-cis RA mitigated 1,25(OH)(2)D3-induced renal calcification by modifying the 1,25(OH)(2)D3-induced increase in ucMGP. The mechanisms by which 9-cis RA and 1,25(OH)(2)D3 alter vitamin K concentrations warrant further investigation.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-(N-methyl-N-nitrosamino)-1-(3-pyri...,
http://linkedlifedata.com/resource/pubmed/chemical/Calcitriol,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Carbon-Carbon Ligases,
http://linkedlifedata.com/resource/pubmed/chemical/Carcinogens,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Matrix Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrosamines,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tretinoin,
http://linkedlifedata.com/resource/pubmed/chemical/Vitamin K,
http://linkedlifedata.com/resource/pubmed/chemical/alitretinoin,
http://linkedlifedata.com/resource/pubmed/chemical/glutamyl carboxylase,
http://linkedlifedata.com/resource/pubmed/chemical/matrix Gla protein
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| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
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| pubmed:issn |
1541-6100
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:volume |
138
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
2337-41
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| pubmed:meshHeading |
pubmed-meshheading:19022954-Animals,
pubmed-meshheading:19022954-Base Sequence,
pubmed-meshheading:19022954-Calcinosis,
pubmed-meshheading:19022954-Calcitriol,
pubmed-meshheading:19022954-Calcium-Binding Proteins,
pubmed-meshheading:19022954-Carbon-Carbon Ligases,
pubmed-meshheading:19022954-Carcinogens,
pubmed-meshheading:19022954-DNA Primers,
pubmed-meshheading:19022954-Dietary Supplements,
pubmed-meshheading:19022954-Extracellular Matrix Proteins,
pubmed-meshheading:19022954-Kidney Diseases,
pubmed-meshheading:19022954-Liver,
pubmed-meshheading:19022954-Male,
pubmed-meshheading:19022954-Mice,
pubmed-meshheading:19022954-Mice, Inbred A,
pubmed-meshheading:19022954-Nitrosamines,
pubmed-meshheading:19022954-RNA, Messenger,
pubmed-meshheading:19022954-Tretinoin,
pubmed-meshheading:19022954-Vitamin K
|
| pubmed:year |
2008
|
| pubmed:articleTitle |
9-Cis retinoic acid reduces 1alpha,25-dihydroxycholecalciferol-induced renal calcification by altering vitamin K-dependent gamma-carboxylation of matrix gamma-carboxyglutamic acid protein in A/J male mice.
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| pubmed:affiliation |
Jean Mayer USDA Human Nutrition Research Center on Aging at Tufts University, Boston, MA 02111, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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