Linked Life Data

19021166

Source:http://linkedlifedata.com/resource/pubmed/id/19021166

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
22
pubmed:dateCreated
2008-12-16
pubmed:abstractText
Yunnan Baiyao (YNBY) is one of the best known traditional Chinese medicines. Saponins are considered to be its active components. In this study, an HPLC method was first developed for the simultaneous quantitative analysis of thirteen saponins, including five triterpenoid saponins and eight steroidal saponins, in a series of YNBY preparations, i. e., powder, capsules, aerosol, toothpaste, plaster, and adhesive bandage. The pre-treatment methods for each dosage form were investigated and optimized. The HPLC separation was performed on a Shim-pack C(18) reversed-phase column in gradient mode with UV detection at 203 nm. All calibration curves showed good linear regression (r(2) > or = 0.9981) within the test ranges. Precisions and repeatabilities of the methods were better than 4.22 and 4.78%, respectively. Recoveries were better than 90.5%, even in the analysis of the least abundant saponins in a complex YNBY plaster. HPLC-ESI-TOF/MS was used for definite identification of compounds in the preparations. This proposed method was successfully applied to quantify the 13 bioactive constituents in 27 commercial samples to evaluate the quality of YNBY preparations. The overall results demonstrate that this method is simple, reliable, and suitable for the quality control of YNBY. Furthermore, the retention behavior of these saponins in reversed-phase chromatography is described.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1615-9314
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3834-46
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Simultaneous quantification of both triterpenoid and steroidal saponins in various Yunnan Baiyao preparations using HPLC-UV and HPLC-MS.
pubmed:affiliation
Key Laboratory of Modern Chinese Medicines, Department of Chinese Materia Medica Analysis, China Pharmaceutical University, Nanjing, China.
pubmed:publicationType
Journal Article, Validation Studies