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rdf:type |
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lifeskim:mentions |
umls-concept:C0006434,
umls-concept:C0021701,
umls-concept:C0023516,
umls-concept:C0026336,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0036974,
umls-concept:C0205082,
umls-concept:C0243072,
umls-concept:C0243076,
umls-concept:C0542341,
umls-concept:C0682002,
umls-concept:C1136310,
umls-concept:C1366562
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pubmed:issue |
2
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pubmed:dateCreated |
2009-2-16
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pubmed:abstractText |
Severe burn shock remains an unsolved clinical problem with urgent needs to explore novel therapeutic approaches. In this study, the in vivo bioactivity of a series of synthetic lactosyl derivatives (oligosaccharides) was assessed on rats with burn shock to elucidate the underlying mechanisms. Administration of An-2 and Gu-4, two lactosyl derivatives with di- and tetravalent beta-D: -galactopyranosyl-(1-4)-beta-D: -glucopyranosyl ligands, significantly prolonged the survival time (P < 0.05 vs. saline), stabilized blood pressure and ameliorated the injuries to vital organs after burn. Flow chamber assay displayed that An-2 and Gu-4 markedly decreased the adhesion of leukocytes to microvessel endothelial cells. Competitive binding assay showed that a CD11b antibody significantly interrupted the interaction of An-2 and Gu-4 with leukocytes from rats with burn shock. With fluorescent microscopy, we further found that the oligosaccharides were selectively bound to leukocytes and with a colocalization of CD11b on the cell membrane. Interestingly, the lectin domain-deficient form of CD11b failed to bind with An-2 and Gu-4. The results suggest that both An-2 and Gu-4 significantly inhibit the adhesion of leukocytes to endothelial cells by binding to CD11b and thereby exert protective effects on severe burn shock.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1573-4986
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pubmed:author |
pubmed-author:DengPengP,
pubmed-author:GongXiaoweiX,
pubmed-author:HeJialuJ,
pubmed-author:JiangYongY,
pubmed-author:LiZhongjunZ,
pubmed-author:LiuAihuaA,
pubmed-author:LiuJinghuaJ,
pubmed-author:StamJ WJW,
pubmed-author:ZhangLinL,
pubmed-author:ZhangShuangquanS,
pubmed-author:ZhaoKesenK,
pubmed-author:ZhaoZhihuiZ
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pubmed:issnType |
Electronic
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
173-88
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pubmed:meshHeading |
pubmed-meshheading:19020974-Animals,
pubmed-meshheading:19020974-Antigens, CD11b,
pubmed-meshheading:19020974-Burns,
pubmed-meshheading:19020974-Cell Adhesion,
pubmed-meshheading:19020974-Disease Models, Animal,
pubmed-meshheading:19020974-Endothelial Cells,
pubmed-meshheading:19020974-Glutamine,
pubmed-meshheading:19020974-Humans,
pubmed-meshheading:19020974-Lactose,
pubmed-meshheading:19020974-Leukocytes,
pubmed-meshheading:19020974-Male,
pubmed-meshheading:19020974-Oligosaccharides,
pubmed-meshheading:19020974-Propanolamines,
pubmed-meshheading:19020974-Rats,
pubmed-meshheading:19020974-Rats, Sprague-Dawley,
pubmed-meshheading:19020974-Shock,
pubmed-meshheading:19020974-Time Factors,
pubmed-meshheading:19020974-Transfection
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pubmed:year |
2009
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pubmed:articleTitle |
Lactosyl derivatives function in a rat model of severe burn shock by acting as antagonists against CD11b of integrin on leukocytes.
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pubmed:affiliation |
Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, Nanjing Normal University, China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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