Linked Life Data

19019725

Source:http://linkedlifedata.com/resource/pubmed/id/19019725

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-12-29
pubmed:abstractText
The resonance light scattering (RLS) spectra of bovine serum albumin (BSA)-dithiothreitol (DTT)-sodium dodecylbenzene sulphonate (SDBS) and its analytical application were investigated. The RLS intensity of this system can be effectively enhanced in the presence of BSA. Based on the enhanced RLS intensity, a simple assay for BSA was developed. The experimental results indicate that the enhanced RLS intensity is proportional to the concentration of BSA in the range from 1.0 x 10(-8) to 7.5 x 10(-7) mol L(-1) with the determination limit of 5.0 x 10(-9) mol L(-1). The effects of pH, concentration of SDBS and DTT on the RLS enhancement were discussed. Most metal ions have little interference on the determination of BSA. Some synthetic and real samples were analyzed, and the results obtained were in good agreement with those obtained by Bradford method.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1386-1425
pubmed:author
pubmed:issnType
Print
pubmed:volume
72
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
178-81
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Determination of protein by resonance light scattering technique using dithiothreitol-sodium dodecylbenzene sulphonate as probe.
pubmed:affiliation
College of Chemistry, Jilin University, Changchun 130012, PR China.
pubmed:publicationType
Journal Article