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rdf:type |
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lifeskim:mentions |
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0028581,
umls-concept:C0185023,
umls-concept:C0237497,
umls-concept:C0331858,
umls-concept:C0443199,
umls-concept:C1171362,
umls-concept:C1314972,
umls-concept:C1515670,
umls-concept:C1704640,
umls-concept:C1706515,
umls-concept:C1708936,
umls-concept:C1947904,
umls-concept:C1999228,
umls-concept:C2825781
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pubmed:issue |
4
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pubmed:dateCreated |
2009-1-23
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pubmed:databankReference |
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pubmed:abstractText |
It is well established that nuclear architecture plays a key role in poising regions of the genome for transcription. This may be achieved using scaffold/matrix attachment regions (S/MARs) that establish loop domains. However, the relationship between changes in the physical structure of the genome as mediated by attachment to the nuclear scaffold/matrix and gene expression is not clearly understood. To define the role of S/MARs in organizing our genome and to resolve the often contradictory loci-specific studies, we have surveyed the S/MARs in HeLa S3 cells on human chromosomes 14-18 by array comparative genomic hybridization. Comparison of LIS (lithium 3,5-diiodosalicylate) extraction to identify SARs and 2 m NaCl extraction to identify MARs revealed that approximately one-half of the sites were in common. The results presented in this study suggest that SARs 5' of a gene are associated with transcript presence whereas MARs contained within a gene are associated with silenced genes. The varied functions of the S/MARs as revealed by the different extraction methods highlights their unique functional contribution.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19017725-10444063,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/19017725-9892199
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1460-2083
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
645-54
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pubmed:dateRevised |
2010-9-23
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pubmed:meshHeading |
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pubmed:year |
2009
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pubmed:articleTitle |
Differential nuclear scaffold/matrix attachment marks expressed genes.
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pubmed:affiliation |
The Center for Molecular Medicine and Genetics, Wayne State University School of Medicine, C.S. Mott Center, Detroit, MI48201, USA.
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pubmed:publicationType |
Journal Article
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