Source:http://linkedlifedata.com/resource/pubmed/id/19016306
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
2009-1-5
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pubmed:abstractText |
Human transcription co-regulator SNW1/SKIP is implicated in the regulation of both transcription elongation and alternative splicing. Prp45, the SNW/SKIP ortholog in yeast, is assumed to be essential for pre-mRNA processing. Here, we characterize prp45(1-169), a temperature sensitive allele of PRP45, which at permissive temperature elicits cell division defects and hypersensitivity to microtubule inhibitors. Using a synthetic lethality screen, we found that prp45(1-169) genetically interacts with alleles of NTC members SYF1, CLF1/SYF3, NTC20, and CEF1, and 2nd step splicing factors SLU7, PRP17, PRP18, and PRP22. Cwc2-associated spliceosomal complexes purified from prp45(1-169) cells showed decreased stoichiometry of Prp22, suggesting its deranged interaction with the spliceosome. In vivo splicing assays in prp45(1-169) cells revealed that branch point mutants accumulated more pre-mRNA whereas 5' and 3' splice site mutants showed elevated levels of lariat-exon intermediate as compared to wild-type cells. Splicing of canonical intron was unimpeded. Notably, the expression of Prp45(119-379) in prp45(1-169) cells restored Prp22 partition in the Cwc2-pulldowns and rescued temperature sensitivity and splicing phenotype of prp45(1-169) strain. Our data suggest that Prp45 contributes, in part through its interaction with the 2nd step-proofreading helicase Prp22, to splicing efficiency of substrates non-conforming to the consensus.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DEAD-box RNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/PRP22 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/PRP45 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/RNA Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1097-4644
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pubmed:author | |
pubmed:copyrightInfo |
2008 Wiley-Liss, Inc.
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pubmed:issnType |
Electronic
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pubmed:day |
1
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pubmed:volume |
106
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
139-51
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:19016306-Alleles,
pubmed-meshheading:19016306-Amino Acid Sequence,
pubmed-meshheading:19016306-DEAD-box RNA Helicases,
pubmed-meshheading:19016306-Introns,
pubmed-meshheading:19016306-Molecular Sequence Data,
pubmed-meshheading:19016306-Mutation,
pubmed-meshheading:19016306-Phenotype,
pubmed-meshheading:19016306-RNA Precursors,
pubmed-meshheading:19016306-RNA Splicing,
pubmed-meshheading:19016306-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:19016306-Spliceosomes
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pubmed:year |
2009
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pubmed:articleTitle |
Prp45 affects Prp22 partition in spliceosomal complexes and splicing efficiency of non-consensus substrates.
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pubmed:affiliation |
Faculty of Science, Department of Cell Biology, Charles University in Prague, Prague 128 00, Czech Republic.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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