Linked Life Data

19014703

Source:http://linkedlifedata.com/resource/pubmed/id/19014703

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PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-12-5
pubmed:abstractText
A simpler way for manipulating droplets on a flat surface was demonstrated, eliminating the complications in the existing methods of open-surface digital microfluidics. Programmed and motorized movements of 10 muL droplets were demonstrated using stepper motors and microcontrollers, including merging, complicated movement along the programmed path, and rapid mixing. Latex immunoagglutination assays for mouse immunoglobulin G, bovine viral diarrhea virus and Escherichia coli were demonstrated by merging two droplets on a superhydrophobic surface (contact angle = 155 +/- 2 degrees ) and using subsequent back light scattering detection, with detection limits of 50 pg mL-1, 2.5 TCID50 mL-1 and 85 CFU mL-1, respectively, all significantly lower than the other immunoassay demonstrations in conventional microfluidics (~1 ng mL-1 for proteins, ~100 TCID50 mL-1 for viruses and ~100 CFU mL-1 for bacteria). Advantages of this system over conventional microfluidics or microwell plate assays include: (1) minimized biofouling and repeated use (>100 times) of a platform; (2) possibility of nanoliter droplet manipulation; (3) reprogrammability with a computer or a game pad interface.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:issn
1754-1611
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
15
pubmed:dateRevised
2011-9-2
pubmed:year
2008
pubmed:articleTitle
Backscattering particle immunoassays in wire-guide droplet manipulations.
pubmed:affiliation
Department of Agricultural and Biosystems Engineering, the University of Arizona, Tucson, AZ 85721-0038, USA. jyyoon@email.arizona.edu.
pubmed:publicationType
Journal Article