Linked Life Data

19010532

Source:http://linkedlifedata.com/resource/pubmed/id/19010532

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2008-12-29
pubmed:abstractText
Plasma polymer surfaces were fabricated such that the cell response to a range of carboxylic acid concentrations on a single sample could be investigated. Surface chemical gradients from hydrophobic plasma polymerised octadiene (OD) to a more hydrophilic plasma polymerised acrylic acid (AA) were formed on glass coverslips. Surface characterisation of the chemical gradients was performed using X-ray photoelectron spectroscopy to determine elemental composition. Following culture of E14 and R1 mouse embryonic stem cells (mES) in differing culture media, cell pluripotency was determined by alkaline phosphatase staining. The results demonstrate that for these cell lines the capacity for self-renewal is maintained if the cells are restricted in their spreading to <120 microm2.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
1878-5905
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
30
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1066-70
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
The geometric control of E14 and R1 mouse embryonic stem cell pluripotency by plasma polymer surface chemical gradients.
pubmed:affiliation
School of Biological Sciences, University of Liverpool, Liverpool L69 7ZB, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't