Linked Life Data

19009452

Source:http://linkedlifedata.com/resource/pubmed/id/19009452

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2008-11-14
pubmed:abstractText
Modern 'metabolomic' methods allow us to compare levels of many structurally diverse compounds in an automated fashion across a large number of samples. This technology is ideally suited to screening of populations of plants, including trials where the aim is the determination of unintended effects introduced by GM. A number of metabolomic methods have been devised for the determination of substantial equivalence. We have developed a methodology, using [1H]-NMR fingerprinting, for metabolomic screening of plants and have applied it to the study of substantial equivalence of field-grown GM wheat. We describe here the principles and detail of that protocol as applied to the analysis of flour generated from field plots of wheat. Particular emphasis is given to the downstream data processing and comparison of spectra by multivariate analysis, from which conclusions regarding metabolome changes due to the GM can be assessed against the background of natural variation due to environment.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
478
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
289-303
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Establishing substantial equivalence: metabolomics.
pubmed:affiliation
National Centre for Plant and Microbial Metabolomics, Rothamsted Research, Harpenden, Hertfordshire, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't