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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
2008-11-13
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pubmed:abstractText |
The prostate cancer HERV-K gag-related NGO-Pr-54 antigen was identified by SEREX analysis using autologous patient serum. NGO-Pr-54 mRNA was observed to be faintly expressed in normal prostate and strongly expressed in a variety of cancers, including ovarian cancer (5/8), prostate cancer (6/9), and leukemia (5/14). A phage plaque assay showed that a strong reaction was constantly observed with clone ZH042 in which the 5' end of NGO-Pr-54 is deleted, suggesting that it contained the sequence coding for the protein product. A TI-35 mAb was produced using a recombinant protein (438 aa) deduced from the sequence of ZH042. Transfection of clone ZH042 into 293T cells resulted in the production of an approximately 50-kDa molecule visualized by Western blotting. Natural production of the molecule was confirmed in a SK-MEL-23 melanoma cell line. An indirect immunofluorescence assay showed that NGO-Pr-54 protein was expressed on the cell surface as well as in the cytoplasm. Cell surface expression was confirmed by flow cytometry using the TI-35 mAb. The antibody response against NGO-Pr-54 was observed in patients with bladder (5.1%), liver (4.1%), lung (3.4%), ovarian (5.6%), and prostate (4.2%) cancer, as well as with malignant melanoma (13.2%).
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1424-9634
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pubmed:author |
pubmed-author:ChayamaKosukeK,
pubmed-author:IshidaToshiakiT,
pubmed-author:MatsushitaHirokazuH,
pubmed-author:MiyamuraTakakoT,
pubmed-author:MorishimaTsuneoT,
pubmed-author:NakamuraYurikaY,
pubmed-author:NakayamaEiichiE,
pubmed-author:ObataYuichiY,
pubmed-author:OharaNobuyaN,
pubmed-author:OldLloyd JLJ,
pubmed-author:SaikaTakashiT,
pubmed-author:SatoShuichiroS,
pubmed-author:UenakaAkikoA,
pubmed-author:WadaHisashiH,
pubmed-author:YamashitaToshiharuT
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pubmed:issnType |
Electronic
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
15
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pubmed:dateRevised |
2010-12-15
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pubmed:meshHeading |
pubmed-meshheading:19006261-Aged, 80 and over,
pubmed-meshheading:19006261-Amino Acid Sequence,
pubmed-meshheading:19006261-Animals,
pubmed-meshheading:19006261-Antibodies, Monoclonal,
pubmed-meshheading:19006261-Antigens, Neoplasm,
pubmed-meshheading:19006261-COS Cells,
pubmed-meshheading:19006261-Cercopithecus aethiops,
pubmed-meshheading:19006261-Cloning, Molecular,
pubmed-meshheading:19006261-Endogenous Retroviruses,
pubmed-meshheading:19006261-Female,
pubmed-meshheading:19006261-Fluorescent Antibody Technique, Indirect,
pubmed-meshheading:19006261-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:19006261-Gene Products, gag,
pubmed-meshheading:19006261-Humans,
pubmed-meshheading:19006261-Immune Sera,
pubmed-meshheading:19006261-Leukemia,
pubmed-meshheading:19006261-Male,
pubmed-meshheading:19006261-Melanoma,
pubmed-meshheading:19006261-Mice,
pubmed-meshheading:19006261-Mice, Inbred BALB C,
pubmed-meshheading:19006261-Molecular Sequence Data,
pubmed-meshheading:19006261-Neoplasm Proteins,
pubmed-meshheading:19006261-Ovarian Neoplasms,
pubmed-meshheading:19006261-Prostate,
pubmed-meshheading:19006261-Prostatic Neoplasms,
pubmed-meshheading:19006261-Recombinant Proteins,
pubmed-meshheading:19006261-Transfection
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pubmed:year |
2008
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pubmed:articleTitle |
Identification of the HERV-K gag antigen in prostate cancer by SEREX using autologous patient serum and its immunogenicity.
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pubmed:affiliation |
Department of Immunology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8558, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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