Linked Life Data

19006204

Source:http://linkedlifedata.com/resource/pubmed/id/19006204

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2009-9-29
pubmed:abstractText
Liquid chromatographic enantiomer separation of several N-benzyloxycarbonyl (CBZ) and N-tert-butoxycarbonyl (BOC) alpha-amino acids and their corresponding ethyl esters was performed on covalently immobilized chiral stationary phases (CSPs) (Chiralpak IA and Chiralpak IB) and coated-type CSPs (Chiralpak AD and Chiralcel OD) based on polysaccharide derivatives. The solvent versatility of the covalently immobilized CSPs in enantiomer separation of N-CBZ and BOC-alpha-amino acids and their ester derivatives was shown and the chromatographic parameters of their enantioselectivities and resolution factors were greatly influenced by the nature of the mobile phase. In general, standard mobile phases using 2-propanol and hexane on Chiralpak IA showed fairly good enantioselectivities for resolution of N-CBZ and BOC-alpha-amino acids and their esters. However, 50% MTBE/hexane (v/v) for resolution of N-CBZ-alpha-amino acids ethyl esters and 20% THF/hexane (v/v) for resolution of N-BOC-alpha-amino acids ethyl esters afforded the greatest enantioselectivities on Chiralpak IA. Also, liquid chromatographic comparisons of the enantiomer resolution of these analytes were made on amylose tris(3,5-dimethylphenylcarbamate)-derived CSPs (Chiralpak IA and Chiralpak AD) and cellulose tris(3,5-dimethylphenylcarbamate)-derived CSPs (Chiralpak IB and Chiralcel OD). Chiralpak AD and/or Chiralcel OD showed the highest enantioselectivities for resolution of N-CBZ-alpha-amino acids and esters, while Chiralpak AD or Chiralpak IA showed the highest resolution of N-BOC-alpha-amino acids and esters.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1520-636X
pubmed:author
pubmed:copyrightInfo
Copyright 2008 Wiley-Liss, Inc.
pubmed:issnType
Electronic
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
871-7
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Comparative studies between covalently immobilized and coated chiral stationary phases based on polysaccharide derivatives for enantiomer separation of N-protected alpha-amino acids and their ester derivatives.
pubmed:affiliation
College of Pharmacy, Chosun University, Gwangju, Republic of Korea.
pubmed:publicationType
Journal Article, Comparative Study