Linked Life Data

19003376

Source:http://linkedlifedata.com/resource/pubmed/id/19003376

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2008-11-12
pubmed:abstractText
The CCE/CC128 cell line, derived from fertilized eggs of theMediterranean fruit fly (Ceratitis capitata), was used toinvestigate whether insect cells in culture could developresistance to Malathion. After 20 cycles of pulse-chasetreatment (28 h exposure to 90 mug/ml of Malathion and 48 hrecovery in normal medium), a Mal 90 selected population wasobtained. DNA content analysis showed that the values were distributed between levels 2C and 4C and no accumulation ofcells in a specific phase of the cell cycle was observed.Furthermore, preliminary molecular analysis showed noamplification of the esterase gene in resistant cells.Cross-resistance of Mal 90 cells towards other insecticides wasassayed and found to be absent. Our data support the idea thatthe medfly cell line and, more generally, insect cell cultures,could represent a promising system to investigate insecticideresistance mechanisms.
pubmed:language
eng
pubmed:journal
pubmed:status
PubMed-not-MEDLINE
pubmed:month
Oct
pubmed:issn
0920-9069
pubmed:author
pubmed:issnType
Print
pubmed:volume
34
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11-5
pubmed:year
2000
pubmed:articleTitle
Induction of Malathion resistance in CCE/CC128 cell line of Mediterranean fruit fly (Ceratitis capitata (Wied.)) (Diptera: Tephritidae).
pubmed:affiliation
Dipartimento Coltivazione e Difesa delle Specie Legnose, Sez. Entomologia Agraria, Via S. Michele degli Scalzi, 2, 56124, Pisa, Italy.
pubmed:publicationType
Journal Article