Source:http://linkedlifedata.com/resource/pubmed/id/19003101
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-3
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| pubmed:dateCreated |
2008-11-12
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| pubmed:abstractText |
Cyclomaltoheptaose (beta-cyclodextrin, beta-CD) is a promising compound for application in various industrial fields because of its ability to entrap various compounds into its hydrophobic cavity. A monoclonal antibody (A7) to beta-CD was generated by using a conjugate of glucosaminylmaltosyl-beta-CD and bovine serum albumin as an antigen. The A7 monoclonal antibody was IgM/kappa and reacted with beta-CD with high specificity. The epitope recognized by the A7 monoclonal antibody seemed to be located on the secondary hydroxyl groups of the rim side of the beta-CD molecule. The dissociation constant of the complex of beta-CD and the immobilized A7 monoclonal antibody was determined to be 1.2 x 10(-4) M. A competitive ELISA using the A7 monoclonal antibody enabled determination of beta-CD and its derivatives with a detection limit of 0.05 muM. This immunoassay was useful to determine beta-CD in biological fluids such as human plasma and urine after appropriate pretreatment of the samples.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:status |
PubMed-not-MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
0920-9069
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
40
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
23-9
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| pubmed:year |
2002
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| pubmed:articleTitle |
A monoclonal antibody to cyclomaltoheptaose (beta-cyclodextrin): Characterization and use for immunoassay of beta-cyclodextrin and its derivatives.
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| pubmed:affiliation |
Department of Applied Biology, Kyoto Institute of Technology, Kyoto, Japan, ikura@ipc.kit.ac.jp.
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| pubmed:publicationType |
Journal Article
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