Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1991-4-8
pubmed:databankReference
pubmed:abstractText
Lactacin F is a heat-stable bacteriocin produced by Lactobacillus acidophilus 11088. A 63-mer oligonucleotide probe deduced from the N-terminal lactacin F amino acid sequence was used to clone the putative laf structural gene from plasmid DNA of a lactacin F-producing transconjugant, L. acidophilus T143. One clone, NCK360, harbored a recombinant plasmid, pTRK160, which contained a 2.2-kb EcoRI fragment of the size expected from hybridization experiments. An Escherichia coli-L. acidophilus shuttle vector was constructed, and a subclone (pTRK162) containing the 2.2-kb EcoRI fragment was introduced by electroporation into two lactacin F-negative strains, L. acidophilus 89 and 88-C. Lactobacillus transformants containing pTRK162 expressed lactacin F activity and immunity. Bacteriocin produced by the transformants exhibited an inhibitory spectrum and heat stability identical to those of the wild-type bacteriocin. An 873-bp region of the 2.2-kb fragment was sequenced by using a 20-mer degenerate lactacin F-specific primer to initiate sequencing from within the lactacin F structural gene. Analysis of the resulting sequence identified an open reading frame which could encode a protein of 75 amino acids. The 25 N-terminal amino acids for lactacin F were identified within the open reading frame along with an N-terminal extension, possibly a signal sequence. The lactacin F N-terminal sequence, through the remainder of the open reading frame (57 amino acids; 6.3 kDa), correlated extremely well with composition analyses of purified lactacin F which also predicted a size of 51 to 56 amino acid residues. Molecular characterization of lactacin F identified a small hydrophobic peptide that may be representative of a common bacteriocin class in lactic acid bacteria.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-1137365, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-13884242, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-16347304, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-1903624, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-2118169, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-2228964, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-2447829, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-2474329, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-2481510, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-2482393, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-2493449, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-2644225, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-2653440, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-2757379, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-2777475, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-2877971, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-3034857, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-3038015, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-3041370, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-3052271, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-3139051, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-3141403, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-3185282, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-3285892, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-3525512, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-3527577, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-364941, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-3733758, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-3883896, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-4598299, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-467675, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-4791308, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-6181373, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-6293930, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-6344016, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-6410990, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-6416164, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-6439113, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-6546423, http://linkedlifedata.com/resource/pubmed/commentcorrection/1900281-791239
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0021-9193
pubmed:author
pubmed:issnType
Print
pubmed:volume
173
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1779-88
pubmed:dateRevised
2010-9-9
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Cloning, phenotypic expression, and DNA sequence of the gene for lactacin F, an antimicrobial peptide produced by Lactobacillus spp.
pubmed:affiliation
Department of Food Science, North Carolina State University, Raleigh 27695-7624.
pubmed:publicationType
Journal Article
More...