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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2008-11-12
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pubmed:abstractText |
Caveolin-1 (Cav-1) plays an important role in modulating cellular signalling, but its role in metastasis is not well defined. A significant reduction in Cav-1 levels was detected in lymph node metastases as compared with primary tumour of head and neck squamous cell carcinoma (HNSCC) specimens (P<0.0001), confirming the downregulation of Cav-1 observed in a highly metastatic M4 cell lines derived from our orthotopic xenograft model. To investigate the function of Cav-1 in metastasis of HNSCC, we compared stable clones of M4 cells carrying human cav-1 cDNA (CavS) with cells expressing an empty vector (EV) in vitro and in the orthotopic xenograft model. Overexpression of Cav-1 suppressed growth of the CavS tumours compared with the EV tumours. The incidence of lung metastases was significantly lower in animals carrying CavS tumours than those with EV tumours (P=0.03). In vitro, CavS cells displayed reduced cell growth, invasion, and increased anoikis compared with EV cells. In CavS cells, Cav-1 formed complex with integrin beta1 and Src. Further application of integrin beta1 neutralising antibody or Src inhibitor PP2 to EV cells illustrated similar phenotypes as CavS cells, suggesting that Cav-1 may play an inhibitory role in tumorigenesis and lung metastasis through regulating integrin beta1- and Src-mediated cell-cell and cell-matrix interactions.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-10825287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-11498016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-11559760,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-11920460,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-11948420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-11997669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-12189159,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-12365014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-12901727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-14996750,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-15331608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-15334058,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-15355971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-15592498,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-15625067,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-15692148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-15802273,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-17028921,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-17123307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-17145853,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-17254967,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-18473353,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-8842478,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19002186-9741627
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1532-1827
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pubmed:author |
pubmed-author:ChenZ GZG,
pubmed-author:HuntJJ,
pubmed-author:MüllerSS,
pubmed-author:RhaS ESE,
pubmed-author:ShinD MDM,
pubmed-author:ShinH J CHJ,
pubmed-author:TaCC,
pubmed-author:TighiouartMM,
pubmed-author:XuZZ,
pubmed-author:ZhangHH,
pubmed-author:ZhangXX
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pubmed:issnType |
Electronic
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pubmed:day |
18
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pubmed:volume |
99
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1684-94
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pubmed:dateRevised |
2010-9-23
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pubmed:meshHeading |
pubmed-meshheading:19002186-Animals,
pubmed-meshheading:19002186-Anoikis,
pubmed-meshheading:19002186-Carcinoma, Squamous Cell,
pubmed-meshheading:19002186-Caveolin 1,
pubmed-meshheading:19002186-Cell Line, Tumor,
pubmed-meshheading:19002186-Cell Proliferation,
pubmed-meshheading:19002186-Disease Models, Animal,
pubmed-meshheading:19002186-Down-Regulation,
pubmed-meshheading:19002186-Female,
pubmed-meshheading:19002186-Head and Neck Neoplasms,
pubmed-meshheading:19002186-Humans,
pubmed-meshheading:19002186-Lymph Nodes,
pubmed-meshheading:19002186-Lymphatic Metastasis,
pubmed-meshheading:19002186-Male,
pubmed-meshheading:19002186-Mice,
pubmed-meshheading:19002186-Mice, Nude,
pubmed-meshheading:19002186-Middle Aged,
pubmed-meshheading:19002186-Neoplasm Metastasis,
pubmed-meshheading:19002186-Xenograft Model Antitumor Assays
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pubmed:year |
2008
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pubmed:articleTitle |
Restoration of caveolin-1 expression suppresses growth and metastasis of head and neck squamous cell carcinoma.
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pubmed:affiliation |
Department of Hematology and Medical Oncology, Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA 30322, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't,
Research Support, N.I.H., Extramural
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