Linked Life Data

19001381

Source:http://linkedlifedata.com/resource/pubmed/id/19001381

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-1-5
pubmed:abstractText
Angiogenesis, the formation of new blood vessels, plays a crucial role in normal physiological processes and in various pathological conditions. In the present study, we have investigated the physiological function of a newly described serine/threonine protein kinase D2 (PKD2) in aspects of endothelial cell biology involved in angiogenesis. We found that PKD2 was expressed in primary human endothelial cells from different tissues and was a critical PKD isoform mediating the phosphorylation of PKD substrates in endothelial cells. By using small interference RNAs that target different PKD2 regions, we found that silencing PKD2, but not PKD1 isoform, markedly inhibited the proliferation, migration, and in vitro angiogenesis of endothelial cells cultured in EGM-2 complete medium. We further showed that PKD2, but not PKD1, was required for the expression of vascular endothelial growth factor receptor-2 and fibroblast growth factor receptor-1 that are two key growth factor receptors involved in angiogenesis. These findings indicate that PKD2 plays a pivotal role in endothelial cell proliferation and migration necessary for angiogenesis at least in part through modulation of the expression of vascular endothelial growth factor receptor-2 and fibroblast growth factor receptor-1.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
9
pubmed:volume
284
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
799-806
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:19001381-Cell Movement, pubmed-meshheading:19001381-Cell Proliferation, pubmed-meshheading:19001381-Cells, Cultured, pubmed-meshheading:19001381-Endothelial Cells, pubmed-meshheading:19001381-Enzyme Activation, pubmed-meshheading:19001381-Humans, pubmed-meshheading:19001381-Isoenzymes, pubmed-meshheading:19001381-Neovascularization, Physiologic, pubmed-meshheading:19001381-Phosphorylation, pubmed-meshheading:19001381-Protein Kinases, pubmed-meshheading:19001381-RNA, Small Interfering, pubmed-meshheading:19001381-Receptor, Fibroblast Growth Factor, Type 1, pubmed-meshheading:19001381-Substrate Specificity, pubmed-meshheading:19001381-Tetradecanoylphorbol Acetate, pubmed-meshheading:19001381-Vascular Endothelial Growth Factor A, pubmed-meshheading:19001381-Vascular Endothelial Growth Factor Receptor-2
pubmed:year
2009
pubmed:articleTitle
Identification of protein kinase D2 as a pivotal regulator of endothelial cell proliferation, migration, and angiogenesis.
pubmed:affiliation
Department of Biochemistry, The University of Texas Health Science Center at Tyler, Tyler, Texas 75708, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't