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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1991-4-2
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pubmed:abstractText |
Virus-transformed pre-B cells undergo ordered immunoglobulin (Ig) gene rearrangements during culture. We devised a series of highly sensitive polymerase chain reaction assays for Ig gene rearrangement and unrearranged Ig gene segment transcription to study both the possible relationship between these processes in cultured pre-B cells and the role played by heavy (H) chain (mu) protein in regulating gene rearrangement. Our analysis of pre-B cell cultures representing various stages of maturity revealed that transcription of each germline Ig locus precedes or is coincident with its rearrangement. Cell lines containing one functional rearranged H chain allele, however, continue to transcribe and to rearrange the allelic, unrearranged H chain locus. These cell lines appear to initiate but not terminate rearrangement events and therefore provide information about the requirements for activating rearrangement but not about allelic exclusion mechanisms.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-168584,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-2505932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-2578321,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-2584932,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-2726754,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-2832182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-3091258,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-3092105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-3096580,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-3098435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-3109035,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-3109454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-3123216,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-3125549,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-3127693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-3158075,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-3265124,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-3317825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-3399892,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-3470759,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-3491327,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-3495343,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-3501371,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-3927301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-3930970,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-4159057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-4160817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-6086308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-6092962,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-6095102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-6277505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-6291785,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-6300689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-6425835,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-6769117,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1900081-6783962
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-1007
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
173
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
711-20
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1900081-Abelson murine leukemia virus,
pubmed-meshheading:1900081-Animals,
pubmed-meshheading:1900081-Base Sequence,
pubmed-meshheading:1900081-Cell Line,
pubmed-meshheading:1900081-Cell Transformation, Viral,
pubmed-meshheading:1900081-Gene Rearrangement,
pubmed-meshheading:1900081-Genes, Immunoglobulin,
pubmed-meshheading:1900081-Humans,
pubmed-meshheading:1900081-Immunoglobulin Constant Regions,
pubmed-meshheading:1900081-Immunoglobulin Heavy Chains,
pubmed-meshheading:1900081-Immunoglobulin Joining Region,
pubmed-meshheading:1900081-Immunoglobulin Light Chains,
pubmed-meshheading:1900081-Immunoglobulin Variable Region,
pubmed-meshheading:1900081-Molecular Sequence Data,
pubmed-meshheading:1900081-Oligonucleotide Probes,
pubmed-meshheading:1900081-Polymerase Chain Reaction,
pubmed-meshheading:1900081-Transcription, Genetic
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pubmed:year |
1991
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pubmed:articleTitle |
Virus-transformed pre-B cells show ordered activation but not inactivation of immunoglobulin gene rearrangement and transcription.
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pubmed:affiliation |
Whitehead Institute, Cambridge, Massachusetts 02142.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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