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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1991-3-27
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pubmed:abstractText |
The extracellular serylprotease subtilisin Carlsberg (SubC) of Bacillus licheniformis is produced in a precursor form which includes a signal peptide (sp) and a pro-region. We have constructed a fusion protein in which the sp, pro-region and 38 amino acids (aa) at the N terminus of SubC were joined to the immunoglobulin (Ig) G-binding protein G produced by group G streptococci. The fused SubC::protein G was purified on IgG-Sepharose. IgG-binding material derived from membrane or supernatant fractions had different N termini, indicating that release from the membrane occurred only after removal of the pro-region. The proteolytic pattern was identical when SubC::protein G was produced in Bacillus subtilis 168 wild type or in a protease-deficient strain. The sp cleavage point was also defined in the membrane-derived material.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0378-1119
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
2
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pubmed:volume |
97
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
49-54
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1899845-Amino Acid Sequence,
pubmed-meshheading:1899845-Bacillus subtilis,
pubmed-meshheading:1899845-Bacterial Proteins,
pubmed-meshheading:1899845-Biological Transport,
pubmed-meshheading:1899845-Blotting, Western,
pubmed-meshheading:1899845-Carrier Proteins,
pubmed-meshheading:1899845-Cell Membrane,
pubmed-meshheading:1899845-Cloning, Molecular,
pubmed-meshheading:1899845-Immunoglobulin G,
pubmed-meshheading:1899845-Molecular Sequence Data,
pubmed-meshheading:1899845-Protein Conformation,
pubmed-meshheading:1899845-Protein Processing, Post-Translational,
pubmed-meshheading:1899845-Recombinant Fusion Proteins,
pubmed-meshheading:1899845-Serine Endopeptidases,
pubmed-meshheading:1899845-Subtilisins
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pubmed:year |
1991
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pubmed:articleTitle |
The subtilisin Carlsberg pro-region is a membrane anchorage for two fusion proteins produced in Bacillus subtilis.
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pubmed:affiliation |
Center for BioTechnology, Karolinska Institute, Huddinge, Sweden.
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pubmed:publicationType |
Journal Article
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