Linked Life Data

18996111

Source:http://linkedlifedata.com/resource/pubmed/id/18996111

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-3
pubmed:dateCreated
2008-12-5
pubmed:abstractText
Clinical trials suggest that synthetic ATP-sensitive K(+) (K(ATP)) channel openers may cause headache and migraine by dilating cerebral and meningeal arteries. We studied the mRNA expression profile of K(ATP) channel subunits in the pig and human middle meningeal artery (MMA) and in the pig middle cerebral artery (MCA). We determined the order of potency of four K(ATP) channel openers when applied to isolated pig MMA and MCA, and we examined the potential inhibitory effects of the Kir6.1 subunit specific K(ATP) channel blocker PNU-37883A on K(ATP) channel opener-induced relaxation of the isolated pig MMA and MCA. Using conventional RT-PCR, we detected the mRNA transcripts of the K(ATP) channel subunits Kir6.1 and SUR2B in all the examined pig and human intracranial arteries. Application of K(ATP) channel openers to isolated pig MMA and MCA in myographs caused a concentration-dependent vasodilatation with an order of potency that supports the presence of functional SUR2B K(ATP) channel subunits. 10(-7) M PNU-37883A significantly inhibited the in vitro dilatory responses of the potent K(ATP) channel opener P-1075 in both pig MMA and MCA. In conclusion, our combined mRNA expression and pharmacological studies indicate that Kir6.1/SUR2B is the major functional K(ATP) channel complex in the pig MMA and MCA, and mRNA expression studies suggest that the human MMA shares this K(ATP) channel subunit profile. Specific blocking of Kir6.1 or SUR2B K(ATP) channel subunits in large cerebral and meningeal arteries may be a future anti-migraine strategy.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1879-0712
pubmed:author
pubmed:issnType
Electronic
pubmed:day
28
pubmed:volume
601
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
43-9
pubmed:meshHeading
pubmed-meshheading:18996111-ATP-Binding Cassette Transporters, pubmed-meshheading:18996111-Adamantane, pubmed-meshheading:18996111-Animals, pubmed-meshheading:18996111-Cerebral Arteries, pubmed-meshheading:18996111-Gene Expression, pubmed-meshheading:18996111-Humans, pubmed-meshheading:18996111-KATP Channels, pubmed-meshheading:18996111-Male, pubmed-meshheading:18996111-Meningeal Arteries, pubmed-meshheading:18996111-Middle Aged, pubmed-meshheading:18996111-Morpholines, pubmed-meshheading:18996111-Potassium Channel Blockers, pubmed-meshheading:18996111-Potassium Channels, Inwardly Rectifying, pubmed-meshheading:18996111-RNA, Messenger, pubmed-meshheading:18996111-Receptors, Drug, pubmed-meshheading:18996111-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:18996111-Species Specificity, pubmed-meshheading:18996111-Swine
pubmed:year
2008
pubmed:articleTitle
K ATP channels in pig and human intracranial arteries.
pubmed:affiliation
Department of Neurology and Danish Headache Center, Glostrup Research Institute, Glostrup Hospital, Faculty of Health Sciences, University of Copenhagen, Denmark. KEBEPL01@glo.regionh.dk
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't