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pubmed-article:1899093pubmed:abstractTextKirsten-ras is the oncogene most frequently activated in human tumors. Studies of its biological function have been limited by the nonavailability of significant amounts of the major protein product, Kirsten-ras (4B) p21. When expressed in Escherichia coli K12, the recombinant protein was rapidly cleaved upon cell lysis in the lysine-rich C terminus region, probably by the ompT protease. However, soluble full-length protein was obtained when the Kirsten-ras gene was expressed in an E. coli strain lacking the ompT gene, and also in a baculovirus/insect cell expression system. Additionally, the baculovirus/insect cell system produced about half of the Kirsten-ras protein in a membrane-associated form, which was post-translationally modified by polyisoprenylation and carboxyl-methylation. A C-terminally truncated form (residues 1-166) was also expressed at high levels in E. coli for x-ray crystallographic studies. The kinetics of GDP release and of GTP hydrolysis of the purified proteins are similar to those of the corresponding Harvey-ras proteins, though there are small differences in the relative affinities for GDP and GTP. Biological activity of full-length Kirsten Val-12 p21 was demonstrated by microinjection into Swiss 3T3 cells, resulting in morphological transformation, with a lower potency than that of Harvey Val-12 protein.lld:pubmed
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pubmed-article:1899093pubmed:pagination1672-8lld:pubmed
pubmed-article:1899093pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:1899093pubmed:articleTitleCharacterization of recombinant human Kirsten-ras (4B) p21 produced at high levels in Escherichia coli and insect baculovirus expression systems.lld:pubmed
pubmed-article:1899093pubmed:affiliationDepartment of Molecular Sciences, Wellcome Research Laboratorie, Langley Court, Beckenham Kent United Kingdom.lld:pubmed
pubmed-article:1899093pubmed:publicationTypeJournal Articlelld:pubmed
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