Linked Life Data

18989972

Source:http://linkedlifedata.com/resource/pubmed/id/18989972

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
2009-2-20
pubmed:abstractText
The retention of phenylalanine ammonia-lyase (PAL) activity in Red Spring wheat seedlings during storage and in vitro protein digestion was evaluated toward assessing the efficacy of plant PAL as a dietary supplement for patients suffering from the metabolic disease, phenylketonuria. Retention of PAL activity in freeze-dried wheat seedling tissues following three months of storage at -20 degrees C ranged from 62% in the leaf to 89% in root/residual seed tissues. After a 3-h two-stage ("gastric-intestinal") in vitro digestion, 36% and 42% recovery of PAL activity was associated with chopped fresh leaf and root/residual seed tissues respectively; however, no activity was recovered from freeze-dried tissues. High performance liquid chromatographic analysis of the residual phenylalanine (Phe) after in vitro digestion confirmed that the fresh tissues effected a significantly higher conversion of exogenous Phe than freeze-dried tissues. These results demonstrate that the plant cell walls provide protection of PAL during in vitro digestion. In cases where exogenous Phe (100 mg; 24 mM) was supplied to the tissues, the product of the reaction, trans-cinnamic acid, may have exerted a significant inhibitory effect on PAL activity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1520-5118
pubmed:author
pubmed:issnType
Electronic
pubmed:day
10
pubmed:volume
56
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11407-12
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Retention of phenylalanine ammonia-lyase activity in wheat seedlings during storage and in vitro digestion.
pubmed:affiliation
University of British Columbia, Vancouver, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't