Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-12-16
pubmed:abstractText
Comprehensive proteomics analyses of spliceosomal complexes are currently limited to those in humans, and thus, it is unclear to what extent the spliceosome's highly complex composition and compositional dynamics are conserved among metazoans. Here we affinity purified Drosophila melanogaster spliceosomal B and C complexes formed in Kc cell nuclear extract. Mass spectrometry revealed that their composition is highly similar to that of human B and C complexes. Nonetheless, a number of Drosophila-specific proteins were identified, suggesting that there may be novel factors contributing specifically to splicing in flies. Protein recruitment and release events during the B-to-C transition were also very similar in both organisms. Electron microscopy of Drosophila B complexes revealed a high degree of structural similarity with human B complexes, indicating that higher-order interactions are also largely conserved. A comparison of Drosophila spliceosomes formed on a short versus long intron revealed only small differences in protein composition but, nonetheless, clear structural differences under the electron microscope. Finally, the characterization of affinity-purified Drosophila mRNPs indicated that exon junction complex proteins are recruited in a splicing-dependent manner during C complex formation. These studies provide insights into the evolutionarily conserved composition and structure of the metazoan spliceosome, as well as its compositional dynamics during catalytic activation.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-10500099, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-10908584, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-11118221, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-11572975, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-11696323, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-11700288, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-11991638, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-12176931, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-12226669, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-12411573, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-12477934, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-12494765, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-12626338, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-12704080, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-12730685, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-12881430, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-12887888, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-12970570, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-1333586, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-14527298, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-14973490, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-14981503, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-1508718, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-15098019, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-15133499, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-15145352, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-15175653, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-15901496, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-15916537, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-16141059, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-16170325, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-16242989, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-16314458, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-16809785, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-16914448, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-17052460, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-17095540, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-17332742, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-17606899, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-18157137, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-18322460, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-18380340, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-2469573, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-2533314, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-3362856, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-3888410, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-6204770, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-6828386, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-7268930, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-7707952, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-7852296, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-8164690, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-8355684, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-8423778, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-8467790, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-8722179, http://linkedlifedata.com/resource/pubmed/commentcorrection/18981222-9476892
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Aptamers, Nucleotide, http://linkedlifedata.com/resource/pubmed/chemical/Cell Extracts, http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Drosophila Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Fushi Tarazu Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins, http://linkedlifedata.com/resource/pubmed/chemical/RNA Precursors, http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoproteins, http://linkedlifedata.com/resource/pubmed/chemical/ftz protein, Drosophila, http://linkedlifedata.com/resource/pubmed/chemical/messenger ribonucleoprotein, http://linkedlifedata.com/resource/pubmed/chemical/z protein, Drosophila
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1098-5549
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
29
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
281-301
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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