Linked Life Data

18981098

Source:http://linkedlifedata.com/resource/pubmed/id/18981098

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
2008-11-4
pubmed:abstractText
Ca(2+)-mediated signal transduction pathways play a central regulatory role in dendritic cell (DC) responses to diverse Ags. However, the mechanisms leading to increased [Ca(2+)](i) upon DC activation remained ill-defined. In the present study, LPS treatment (100 ng/ml) of mouse DCs resulted in a rapid increase in [Ca(2+)](i), which was due to Ca(2+) release from intracellular stores and influx of extracellular Ca(2+) across the cell membrane. In whole-cell voltage-clamp experiments, LPS-induced currents exhibited properties similar to the currents through the Ca(2+) release-activated Ca(2+) channels (CRAC). These currents were highly selective for Ca(2+), exhibited a prominent inward rectification of the current-voltage relationship, and showed an anomalous mole fraction and a fast Ca(2+)-dependent inactivation. In addition, the LPS-induced increase of [Ca(2+)](i) was sensitive to margatoxin and ICAGEN-4, both inhibitors of voltage-gated K(+) (Kv) channels Kv1.3 and Kv1.5, respectively. MHC class II expression, CCL21-dependent migration, and TNF-alpha and IL-6 production decreased, whereas phagocytic capacity increased in LPS-stimulated DCs in the presence of both Kv channel inhibitors as well as the I(CRAC) inhibitor SKF-96365. Taken together, our results demonstrate that Ca(2+) influx in LPS-stimulated DCs occurs via Ca(2+) release-activated Ca(2+) channels, is sensitive to Kv channel activity, and is in turn critically important for DC maturation and functions.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1550-6606
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
181
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6803-9
pubmed:meshHeading
pubmed-meshheading:18981098-Animals, pubmed-meshheading:18981098-Calcium Channels, pubmed-meshheading:18981098-Cell Differentiation, pubmed-meshheading:18981098-Cell Movement, pubmed-meshheading:18981098-Dendritic Cells, pubmed-meshheading:18981098-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:18981098-Female, pubmed-meshheading:18981098-Flow Cytometry, pubmed-meshheading:18981098-Immunohistochemistry, pubmed-meshheading:18981098-Interleukin-6, pubmed-meshheading:18981098-Lipopolysaccharides, pubmed-meshheading:18981098-Membrane Potentials, pubmed-meshheading:18981098-Mice, pubmed-meshheading:18981098-Patch-Clamp Techniques, pubmed-meshheading:18981098-Potassium Channels, Voltage-Gated, pubmed-meshheading:18981098-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:18981098-Tumor Necrosis Factor-alpha
pubmed:year
2008
pubmed:articleTitle
Ion channels modulating mouse dendritic cell functions.
pubmed:affiliation
Department of Physiology, University of Tübingen, Gmelinstr. 5, Tübingen, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't