Linked Life Data

18980207

Source:http://linkedlifedata.com/resource/pubmed/id/18980207

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-12-16
pubmed:abstractText
The neoplastic environment is generally regarded as an immunosuppressive milieu. However, a group of cancers are characterized by the abundance of tumour-infiltrating lymphocytes (TILs). Here we examined the possible roles of chemokines in the formation of lymphoid stroma in lymphocyte-rich gastric carcinomas (GCs), including EBV(+) cases and conventional GCs. Regardless of EBV positivity, TILs in lymphocyte-rich GCs predominantly expressed CXCR3, while its ligand CXCL9 was abundantly expressed by stromal cells and a portion of cancer cells. CXCL9(+) stromal cells were judged to include dendritic cells, because they partly co-expressed fascin, DC-sign, CD83, DC-lamp or HLA-DR. T cells in close contact with CXCL9(+) cells showed frequent labelling of Ki-67 (approximately 10%), suggesting the immunostimulatory activity of CXCL9(+) stromal cells. The T-cell zone of the regional lymph nodes of lymphocyte-rich GCs also abounded with CXCR3(+) T cells and CXCL9(+) stromal cells. This indicated a close similarity between cancer stroma and regional lymph nodes of lymphocyte-rich GCs. Quantitative RT-PCR also confirmed the strong expression of CXCR3, CXCL9 and IFNgamma in lymphocyte-rich GCs. In contrast, conventional GCs contained less abundant CXCR3(+) T cells and few CXCL9(+) stromal cells. Collectively, the CXCL9-CXCR3 axis plays a pivotal role in the formation of lymphoid stroma in lymphocyte-rich GCs. Given similar findings in the regional lymph nodes, the lymphoid stroma of lymphocyte-rich GCs may represent a tertiary lymphoid tissue with predominantly Th1-shifted immune responses.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1096-9896
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
217
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
21-31
pubmed:meshHeading
pubmed-meshheading:18980207-Cell Proliferation, pubmed-meshheading:18980207-Chemokine CXCL10, pubmed-meshheading:18980207-Chemokine CXCL9, pubmed-meshheading:18980207-Dendritic Cells, pubmed-meshheading:18980207-Epstein-Barr Virus Infections, pubmed-meshheading:18980207-Forkhead Transcription Factors, pubmed-meshheading:18980207-Humans, pubmed-meshheading:18980207-Immunoenzyme Techniques, pubmed-meshheading:18980207-Interferon-gamma, pubmed-meshheading:18980207-Lymph Nodes, pubmed-meshheading:18980207-Lymphocytes, Tumor-Infiltrating, pubmed-meshheading:18980207-Neoplasm Staging, pubmed-meshheading:18980207-RNA, Messenger, pubmed-meshheading:18980207-Receptors, CXCR3, pubmed-meshheading:18980207-Stomach Neoplasms, pubmed-meshheading:18980207-Stromal Cells, pubmed-meshheading:18980207-T-Lymphocyte Subsets, pubmed-meshheading:18980207-T-Lymphocytes, Regulatory
pubmed:year
2009
pubmed:articleTitle
Abundant expression of CXCL9 (MIG) by stromal cells that include dendritic cells and accumulation of CXCR3+ T cells in lymphocyte-rich gastric carcinoma.
pubmed:affiliation
Department of Pathology, Mito Medical Centre, National Hospital Organization, Ibaraki, Japan. hohtani@mito.hosp.go.jp
pubmed:publicationType
Journal Article, Multicenter Study