Linked Life Data

18980124

Source:http://linkedlifedata.com/resource/pubmed/id/18980124

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2008-11-4
pubmed:abstractText
A highly sensitive and selective method that requires minimal sample preparation was developed for the confirmation and quantitation of cyclamate in a variety of foods by high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS). Sample preparation consisted of homogenization followed by extraction and dilution of cyclamate with water. HPLC separation was achieved using a bridged ethyl hybrid C18 high-pressure column with a mobile phase consisting of 0.15% acetic acid and methanol. Under electrospray ionization negative conditions, quantitation was achieved by monitoring the fragment m/z = 79.7 while also collecting parent ion m/z = 177.9. Two food matrixes, diet soda and jelly, were subjected to a validation procedure in order to evaluate the applicability of the method. The cyclamate limit of detection for both matrixes was determined to be 0.050 microg/g with a limit of quantitation of 0.150 microg/g. The correlation coefficient of the calibration curves was >0.9998 from 0.0005 to 0.100 microg/mL. The method has been used for the determination of cyclamate in several foods and the results are presented.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1060-3271
pubmed:author
pubmed:issnType
Print
pubmed:volume
91
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1095-102
pubmed:meshHeading
pubmed:articleTitle
Determination of cyclamate in foods by ultraperformance liquid chromatography/tandem mass spectrometry.
pubmed:affiliation
New York State Department of Agriculture and Markets, Food Laboratory, Building 7, State Office Campus, Albany, NY 12235, USA. Robert.sheridan@agmkt.state.ny.us
pubmed:publicationType
Journal Article