Linked Life Data

1895928

Source:http://linkedlifedata.com/resource/pubmed/id/1895928

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1991-10-21
pubmed:abstractText
To determine the role of hemolysin(s) in virulence and immunoprotection, non-hemolytic mutants of Actinobacillus pleuropneumoniae serotype 5, strain J45, were isolated following chemical mutagenesis. One mutant was selected for extensive characterization. Differences in capsule content, or in lipopolysaccharide or membrane protein electrophoretic profiles of the parent and mutant were not detected. A predominant, calcium-inducible protein of 110 kDa was present in culture supernatant of the parent, but absent from the mutant. Two-dimensional (2-D) gel electrophoresis confirmed that the 110 kDa protein was absent in culture supernatant of the mutant, but few, if any, minor differences could be detected in whole-cell proteins between the parent and mutant. The mutant totally lacked extracellular hemolytic and cytotoxic activity. Lysates of whole cells of the mutant contained weak hemolytic activity, and the 110 kDa protein could be detected by immunoblotting. Neutralization titers were negative in pigs immunized with the mutant or purified, denatured hemolysin, although enzyme-immunoassay titers were detected. Four additional independently isolated non-hemolytic mutants were avirulent in pigs and mice at doses greater than 10 times the lethal dose of the parent. Neither pigs nor mice were protected against lethal infection following immunization with the non-hemolytic mutant. We conclude that the 110 kDa hemolysin plays an important role in bacterial virulence and the pathogenesis of pleuropneumonia, and that sufficiently high levels of neutralizing antibodies to the 110 kDa hemolysin may be required for protection of pigs against disease.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0882-4010
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
281-96
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Characterization of a non-hemolytic mutant of Actinobacillus pleuropneumoniae serotype 5: role of the 110 kilodalton hemolysin in virulence and immunoprotection.
pubmed:affiliation
Department of Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, Blacksburg 24061.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't