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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1991-10-21
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pubmed:abstractText |
Phospholipase D (EC 3.1.4.4) of Streptomyces species was found to catalyze transphosphatidylation to L-ascorbic acid from phosphatidylcholine (PC) in a biphasic reaction system. The product was identified as 1,2-diacyl-sn-glycero-3-phospho-6'-L-ascorbic acid (PA-AsA) by mass spectrometry and nuclear magnetic resonance spectroscopy. The optimal pH of transphosphatidylation was 4.5 and the rate of PA-AsA formation increased as concentrations of L-ascorbic acid increased. The conversion of PC to PA-AsA was greater than 80%. PA-AsA was found to be more resistant to hydrolysis by phospholipase D than was PC.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0024-4201
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
390-4
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pubmed:dateRevised |
2003-11-14
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pubmed:meshHeading |
pubmed-meshheading:1895887-Ascorbic Acid,
pubmed-meshheading:1895887-Glycerophospholipids,
pubmed-meshheading:1895887-Hydrogen-Ion Concentration,
pubmed-meshheading:1895887-Kinetics,
pubmed-meshheading:1895887-Magnetic Resonance Spectroscopy,
pubmed-meshheading:1895887-Phosphatidic Acids,
pubmed-meshheading:1895887-Phospholipase D,
pubmed-meshheading:1895887-Spectrometry, Mass, Fast Atom Bombardment,
pubmed-meshheading:1895887-Streptomyces,
pubmed-meshheading:1895887-Time Factors
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pubmed:year |
1991
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pubmed:articleTitle |
Synthesis of 6-phosphatidyl-L-ascorbic acid by phospholipase D.
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pubmed:affiliation |
National Food Research Institute, Ministry of Agriculture, Forestry and Fisheries, Ibaraki, Japan.
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pubmed:publicationType |
Journal Article
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