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rdf:type |
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lifeskim:mentions |
|
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pubmed:issue |
11
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pubmed:dateCreated |
2008-10-31
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pubmed:databankReference |
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pubmed:abstractText |
A common application of fluorescent proteins is to label whole cells, but many RFPs are cytotoxic when used with standard high-level expression systems. We engineered a rapidly maturing tetrameric fluorescent protein called DsRed-Express2 that has minimal cytotoxicity. DsRed-Express2 exhibits strong and stable expression in bacterial and mammalian cells, and it outperforms other available RFPs with regard to photostability and phototoxicity.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
|
|
pubmed:citationSubset |
IM
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|
pubmed:chemical |
|
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1548-7105
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
955-7
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pubmed:dateRevised |
2011-6-1
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pubmed:meshHeading |
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|
pubmed:year |
2008
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pubmed:articleTitle |
A noncytotoxic DsRed variant for whole-cell labeling.
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pubmed:affiliation |
Department of Biochemistry and Molecular Biology, The University of Chicago, Gordon Center W238, Chicago, Illinois 60637, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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