Linked Life Data

18952091

Source:http://linkedlifedata.com/resource/pubmed/id/18952091

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2008-12-22
pubmed:abstractText
The invasion of red blood cells (RBCs) is an essential event in the life cycle of all malaria-causing Plasmodium parasites; however, there are major gaps in our knowledge of this process. Here, we use video microscopy to address the kinetics of RBC invasion in the human malaria parasite Plasmodium falciparum. Under in vitro conditions merozoites generally recognise new target RBCs within 1 min of their release from their host RBC. Parasite entry ensues and is complete on average 27.6s after primary contact. This period can be divided into two distinct phases. The first is an approximately 11s 'pre-invasion' phase that involves an often dramatic RBC deformation and recovery process. The second is the classical 'invasion' phase where the merozoite becomes internalised within the RBC in a approximately 17s period. After invasion, a third 'echinocytosis' phase commences when about 36 s after every successful invasion a dramatic dehydration-type morphology was adopted by the infected RBC. During this phase, the echinocytotic effect reached a peak over the next 23.4s, after which the infected RBC recovered over a 5-11 min period. By then the merozoite had assumed an amoeboid-like state and was apparently free in the cytoplasm. A comparison of our data with that of an earlier study of the distantly related primate parasite Plasmodium knowlesi indicated remarkable similarities, suggesting that the kinetics of invasion are conserved across the Plasmodium genus. This study provides a morphological and kinetic framework onto which the invasion-associated physiological and molecular events can be overlaid.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1879-0135
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
91-6
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Morphology and kinetics of the three distinct phases of red blood cell invasion by Plasmodium falciparum merozoites.
pubmed:affiliation
Walter and Eliza Hall Institute of Medical Research, Parkville, Vic. 3050, Australia. gilson@burnet.edu.au
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't