Linked Life Data

18945507

Source:http://linkedlifedata.com/resource/pubmed/id/18945507

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2008-11-17
pubmed:abstractText
The RNA-binding properties of the p17 protein of Dendrolimus punctatus tetravirus were analysed. We have demonstrated that p17 protein, a nonstructural protein with a potentially important role in viral replication, is a RNA-binding protein, which has not been previously described for any member of the family Tetraviridae. P17 protein was expressed in Escherichia coli and was used in UV cross-linking analysis, using a digoxigenin-UTP-labeled RNA probe and chemical cross-linking analysis. The analyses demonstrated that p17 protein could bind to RNA. When analysed for capacity of p17 to form oligomers, the protein could form dimers and tetramers. Furthermore, the circular dichroism spectrums of viral RNA 3'-UTR proved that their secondary structures were consistent with yeast tRNA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0168-1702
pubmed:author
pubmed:issnType
Print
pubmed:volume
138
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1-6
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
RNA-binding properties of Dendrolimus punctatus tetravirus p17 protein.
pubmed:affiliation
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, Hubei 430072, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't