Linked Life Data

18941190

Source:http://linkedlifedata.com/resource/pubmed/id/18941190

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2008-10-22
pubmed:abstractText
Stem cell transplantation across HLA barriers may trigger NK cell-mediated graft-vs-leukemia effects leading to improved survival for patients with hematological malignancies. However, the genetic algorithm based on killer cell Ig-like receptor (KIR) and HLA genes used to predict NK cell alloreactivity have yielded discrepant results. Accordingly, it has been difficult to define transplantation settings that favor NK cell alloreactivity. In this study, we have used multiparameter flow cytometry to simultaneously analyze the cell surface expression of all four major inhibitory KIR and CD94/NKG2A to determine the size of the alloreactive NK cell repertoires in 31 individuals homozygous for the group A KIR haplotype. We observed a vast variability in the frequencies of cells with an alloreactive potential, ranging from 0 to 62% of the total NK cell population depending on which, and how many, KIR ligands were missing in theoretical recipients. This analysis required a functional examination of KIR3DL2-single positive NK cells, showing that this subset was hyporesponsive in individuals harboring the cognate ligands HLA-A3/A11. The results provide new insights into the variability of the functional alloreactive NK cell repertoire and have implications for donor selection in hematopoietic stem cell transplantation and adoptive NK cell-based immunotherapy.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1550-6606
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
181
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
6010-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:18941190-Antibodies, Monoclonal, pubmed-meshheading:18941190-Cytotoxicity Tests, Immunologic, pubmed-meshheading:18941190-Genotype, pubmed-meshheading:18941190-Graft vs Leukemia Effect, pubmed-meshheading:18941190-HLA-A Antigens, pubmed-meshheading:18941190-HLA-A11 Antigen, pubmed-meshheading:18941190-HLA-A3 Antigen, pubmed-meshheading:18941190-Haplotypes, pubmed-meshheading:18941190-Histocompatibility Testing, pubmed-meshheading:18941190-Homozygote, pubmed-meshheading:18941190-Humans, pubmed-meshheading:18941190-K562 Cells, pubmed-meshheading:18941190-Killer Cells, Natural, pubmed-meshheading:18941190-Ligands, pubmed-meshheading:18941190-NK Cell Lectin-Like Receptor Subfamily C, pubmed-meshheading:18941190-Receptors, KIR3DL2, pubmed-meshheading:18941190-Stem Cell Transplantation
pubmed:year
2008
pubmed:articleTitle
Estimation of the size of the alloreactive NK cell repertoire: studies in individuals homozygous for the group A KIR haplotype.
pubmed:affiliation
Center for Infectious Medicine, Department of Medicine, Karolinska Institutet, Karolinska University Hospital, Stockholm, Sweden.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't