Source:http://linkedlifedata.com/resource/pubmed/id/18931650
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
12
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| pubmed:dateCreated |
2008-11-21
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| pubmed:abstractText |
Aurora-A kinase is a cell-cycle-regulating kinase required for chromosomal segregation. Overexpression of Aurora-A kinase has been shown to correlate with tumor proliferation and chromosomal instability. We investigated Aurora-A kinase expression in peripheral blood and bone marrow of 47 chronic lymphocytic leukemia patients and 20 age-matched hematologically healthy subjects. Western blot analysis showed significantly higher Aurora-A levels in chronic lymphocytic leukemia (42 of 47) compared with lymphocytes of healthy subjects. However, Aurora-A mRNA expression in three chronic lymphocytic leukemia patients was similar to or lower than that of healthy control subjects. In 28 of 42 chronic lymphocytic leukemia patients with elevated Aurora-A kinase expression, one or more chromosomal abnormalities were detected, including trisomy 12 in 9 patients and deletion of the ataxia telangiectasia-mutated gene in 9 patients. Aurora-A was also detected in all (100%) chronic lymphocytic leukemia cases by immunohistochemistry, with a nuclear staining pattern. The larger prolymphocytes and paraimmunoblasts showed stronger Aurora-A kinase expression than did small lymphocytes. In contrast, normal bone marrow reactive lymphocytes were negative for Aurora-A with positive histiocytes and immature myeloid cells. Immunostaining for acetylated histone H3 showed a nuclear pattern in all 38 chronic lymphocytic leukemia cases and double labeling showed coexpression of acetylated histone H3 and Aurora-A. In summary, Aurora-A kinase is overexpressed in chronic lymphocytic leukemia cells. The expression of acetylated histone H3 suggests that Aurora-A kinase may be active (functional). Thus, Aurora-A kinase overexpression in chronic lymphocytic leukemia may be involved in the genesis of chromosomal abnormalities and is a potential target for therapeutic intervention.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Histones,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53,
http://linkedlifedata.com/resource/pubmed/chemical/aurora kinase
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| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
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| pubmed:issn |
1530-0285
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| pubmed:author |
pubmed-author:Bueso-RamosCarlos ECE,
pubmed-author:FernandezMichaelM,
pubmed-author:InamdarKedar VKV,
pubmed-author:KeatingMichaelM,
pubmed-author:MedeirosL JeffreyLJ,
pubmed-author:NguyenMartin HMH,
pubmed-author:O'BrienSusanS,
pubmed-author:SenSubrataS,
pubmed-author:ThomazyVilmosV,
pubmed-author:WangXuemeiX
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| pubmed:issnType |
Electronic
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| pubmed:volume |
21
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1428-35
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| pubmed:dateRevised |
2011-7-11
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| pubmed:meshHeading |
pubmed-meshheading:18931650-Acetylation,
pubmed-meshheading:18931650-Adult,
pubmed-meshheading:18931650-Aged,
pubmed-meshheading:18931650-Aged, 80 and over,
pubmed-meshheading:18931650-Blotting, Western,
pubmed-meshheading:18931650-Cell Nucleus,
pubmed-meshheading:18931650-Chromosome Aberrations,
pubmed-meshheading:18931650-Female,
pubmed-meshheading:18931650-Flow Cytometry,
pubmed-meshheading:18931650-Histones,
pubmed-meshheading:18931650-Humans,
pubmed-meshheading:18931650-Immunohistochemistry,
pubmed-meshheading:18931650-In Situ Hybridization, Fluorescence,
pubmed-meshheading:18931650-Leukemia, Lymphocytic, Chronic, B-Cell,
pubmed-meshheading:18931650-Male,
pubmed-meshheading:18931650-Middle Aged,
pubmed-meshheading:18931650-Protein-Serine-Threonine Kinases,
pubmed-meshheading:18931650-RNA, Messenger,
pubmed-meshheading:18931650-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:18931650-Tumor Suppressor Protein p53
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| pubmed:year |
2008
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| pubmed:articleTitle |
Aurora-A kinase nuclear expression in chronic lymphocytic leukemia.
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| pubmed:affiliation |
Department of Hematopathology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USA.
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| pubmed:publicationType |
Journal Article
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