Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9-10
pubmed:dateCreated
2008-12-8
pubmed:abstractText
The aminoglycoside resistance genes sgm from Micromonospora zionensis and kgmB from Streptomyces tenebrarius were cloned into a yeast expression vector to test whether the encoded prokaryotic methylases can modify the 18S rRNA A-site and thus confer resistance to G-418. Despite the detectable presence of mRNAs in yeast cells, neither G-418-resistant yeast transformants nor positive western blot signals were obtained. Neither methylase was capable of methylating 40S subunits despite very high conservation of the antibiotic rRNA binding sites. However, the results provide novel insight into the action of Sgm by showing that it methylates the same site as KgmB, i.e. G1405 in 16S rRNA.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/18930134-12962310, http://linkedlifedata.com/resource/pubmed/commentcorrection/18930134-1447159, http://linkedlifedata.com/resource/pubmed/commentcorrection/18930134-15578257, http://linkedlifedata.com/resource/pubmed/commentcorrection/18930134-15663932, http://linkedlifedata.com/resource/pubmed/commentcorrection/18930134-16540698, http://linkedlifedata.com/resource/pubmed/commentcorrection/18930134-2441068, http://linkedlifedata.com/resource/pubmed/commentcorrection/18930134-3038686, http://linkedlifedata.com/resource/pubmed/commentcorrection/18930134-3058329, http://linkedlifedata.com/resource/pubmed/commentcorrection/18930134-3655737, http://linkedlifedata.com/resource/pubmed/commentcorrection/18930134-7585249, http://linkedlifedata.com/resource/pubmed/commentcorrection/18930134-8808941
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0923-2508
pubmed:author
pubmed:issnType
Print
pubmed:volume
159
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
658-62
pubmed:dateRevised
2010-9-21
pubmed:meshHeading
pubmed:articleTitle
Aminoglycoside resistance genes sgm and kgmB protect bacterial but not yeast small ribosomal subunits in vitro despite high conservation of the rRNA A-site.
pubmed:affiliation
Institute of Molecular Genetics and Genetic Engineering, Vojvode Stepe 444a, P.O. Box 23, 11010 Beograd, Serbia. tatjanai@eunet.yu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't