Linked Life Data

18924136

Source:http://linkedlifedata.com/resource/pubmed/id/18924136

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2008-12-2
pubmed:abstractText
In this review, which is more or less a transcript of my du Vigneaud Award Lecture, I cover the development and application of the protein semisynthesis technique, Expressed Protein Ligation (EPL). EPL allows the assembly of modified proteins from recombinant and synthetic peptide building blocks. The approach has been widely used since its introduction in 1998 and has allowed a number of biochemical problems to be solved through the use of CEdesigner proteins. In this article, the utility of the approach is illustrated through work in my own lab and with an emphasis on the use of EPL to study the role of protein post-translational modifications.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0006-3525
pubmed:author
pubmed:copyrightInfo
Copyright 2008 Wiley Periodicals, Inc.
pubmed:issnType
Print
pubmed:volume
90
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
743-50
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Studying protein structure and function using semisynthesis.
pubmed:affiliation
Laboratory of Synthetic Protein Chemistry, The Rockefeller University, 1230 York Avenue, New York, NY 10021, USA. muirt@mail.rockefeller.edu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Research Support, N.I.H., Extramural