| pubmed-article:1888735 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1888735 | lifeskim:mentions | umls-concept:C0012939 | lld:lifeskim |
| pubmed-article:1888735 | lifeskim:mentions | umls-concept:C0040669 | lld:lifeskim |
| pubmed-article:1888735 | lifeskim:mentions | umls-concept:C1704689 | lld:lifeskim |
| pubmed-article:1888735 | lifeskim:mentions | umls-concept:C0242958 | lld:lifeskim |
| pubmed-article:1888735 | lifeskim:mentions | umls-concept:C0221198 | lld:lifeskim |
| pubmed-article:1888735 | lifeskim:mentions | umls-concept:C0079866 | lld:lifeskim |
| pubmed-article:1888735 | lifeskim:mentions | umls-concept:C0596040 | lld:lifeskim |
| pubmed-article:1888735 | lifeskim:mentions | umls-concept:C0205250 | lld:lifeskim |
| pubmed-article:1888735 | lifeskim:mentions | umls-concept:C1549810 | lld:lifeskim |
| pubmed-article:1888735 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:1888735 | pubmed:issue | 36 | lld:pubmed |
| pubmed-article:1888735 | pubmed:dateCreated | 1991-10-17 | lld:pubmed |
| pubmed-article:1888735 | pubmed:abstractText | It is widely accepted that mutagenic DNA lesions fall into two categories: mispairing lesions hydrogen bond with an incorrect incoming base, generally do not stop replication, and possess high mutagenic efficiency without any requirement for induced functions; noninstructional lesions lack accessible template information, act as strong blocks to DNA replication (and are therefore toxic), and their mutagenic effects are SOS-dependent. Our recent results show that ethenocytosine (epsilon C), a noninstructional exocyclic DNA lesion induced by vinyl chloride, may have unusual mutagenic properties. To obtain more definitive experimental evidence for the observed effects, we have introduced a single epsilon C residue at a specific site of coliphage M13AB28 replicative form DNA by a "single-stranded linker-ligation" technique. The resulting DNA was purified and transfected into appropriate recA+ or recA- Escherichia coli host cells. The effect of epsilon C on survival was determined from transfection efficiency. Both the frequency and specificity of mutations induced by epsilon C were determined by direct sequence analysis of randomly picked progeny phage plaques. The results indicated that epsilon C has little effect on the survival of M13 DNA. Approximately 30% of the progeny phage obtained by transfecting epsilon C DNA had a base substitution mutation precisely at the lesion site. No such mutations were observed in progeny plaques obtained by transfecting the control DNA construct. All epsilon C-induced mutations were either C-to-T transitions or C-to-A transversions. Neither survival nor mutagenic efficiency was significantly affected in recA- host cells.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:1888735 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1888735 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1888735 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1888735 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1888735 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1888735 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1888735 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1888735 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1888735 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1888735 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1888735 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1888735 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1888735 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:1888735 | pubmed:issn | 0006-2960 | lld:pubmed |
| pubmed-article:1888735 | pubmed:author | pubmed-author:HumayunM ZMZ | lld:pubmed |
| pubmed-article:1888735 | pubmed:author | pubmed-author:SimhaDD | lld:pubmed |
| pubmed-article:1888735 | pubmed:author | pubmed-author:PalejwalaV... | lld:pubmed |
| pubmed-article:1888735 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1888735 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:1888735 | pubmed:volume | 30 | lld:pubmed |
| pubmed-article:1888735 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1888735 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1888735 | pubmed:pagination | 8736-43 | lld:pubmed |
| pubmed-article:1888735 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:1888735 | pubmed:meshHeading | pubmed-meshheading:1888735-... | lld:pubmed |
| pubmed-article:1888735 | pubmed:meshHeading | pubmed-meshheading:1888735-... | lld:pubmed |
| pubmed-article:1888735 | pubmed:meshHeading | pubmed-meshheading:1888735-... | lld:pubmed |
| pubmed-article:1888735 | pubmed:meshHeading | pubmed-meshheading:1888735-... | lld:pubmed |
| pubmed-article:1888735 | pubmed:meshHeading | pubmed-meshheading:1888735-... | lld:pubmed |
| pubmed-article:1888735 | pubmed:meshHeading | pubmed-meshheading:1888735-... | lld:pubmed |
| pubmed-article:1888735 | pubmed:meshHeading | pubmed-meshheading:1888735-... | lld:pubmed |
| pubmed-article:1888735 | pubmed:meshHeading | pubmed-meshheading:1888735-... | lld:pubmed |
| pubmed-article:1888735 | pubmed:meshHeading | pubmed-meshheading:1888735-... | lld:pubmed |
| pubmed-article:1888735 | pubmed:meshHeading | pubmed-meshheading:1888735-... | lld:pubmed |
| pubmed-article:1888735 | pubmed:meshHeading | pubmed-meshheading:1888735-... | lld:pubmed |
| pubmed-article:1888735 | pubmed:meshHeading | pubmed-meshheading:1888735-... | lld:pubmed |
| pubmed-article:1888735 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1888735 | pubmed:articleTitle | Mechanisms of mutagenesis by exocyclic DNA adducts. Transfection of M13 viral DNA bearing a site-specific adduct shows that ethenocytosine is a highly efficient RecA-independent mutagenic noninstructional lesion. | lld:pubmed |
| pubmed-article:1888735 | pubmed:affiliation | Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey-New Jersey Medical School, Newark 07103-2714. | lld:pubmed |
| pubmed-article:1888735 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1888735 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1888735 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1888735 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1888735 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1888735 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1888735 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1888735 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1888735 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1888735 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1888735 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1888735 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1888735 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1888735 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:1888735 | lld:pubmed |
