| pubmed-article:1888734 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:1888734 | lifeskim:mentions | umls-concept:C1704689 | lld:lifeskim |
| pubmed-article:1888734 | lifeskim:mentions | umls-concept:C0028953 | lld:lifeskim |
| pubmed-article:1888734 | lifeskim:mentions | umls-concept:C0242958 | lld:lifeskim |
| pubmed-article:1888734 | lifeskim:mentions | umls-concept:C1521970 | lld:lifeskim |
| pubmed-article:1888734 | lifeskim:mentions | umls-concept:C0079866 | lld:lifeskim |
| pubmed-article:1888734 | lifeskim:mentions | umls-concept:C1527177 | lld:lifeskim |
| pubmed-article:1888734 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:1888734 | lifeskim:mentions | umls-concept:C0441513 | lld:lifeskim |
| pubmed-article:1888734 | lifeskim:mentions | umls-concept:C1705542 | lld:lifeskim |
| pubmed-article:1888734 | lifeskim:mentions | umls-concept:C0205171 | lld:lifeskim |
| pubmed-article:1888734 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:1888734 | pubmed:issue | 36 | lld:pubmed |
| pubmed-article:1888734 | pubmed:dateCreated | 1991-10-17 | lld:pubmed |
| pubmed-article:1888734 | pubmed:abstractText | By using a gene-targeted random DNA adduction approach, we have recently shown that chloroacetaldehyde, a metabolite of vinyl chloride, induces mutations predominantly at cytosines under conditions in which both ethenoadenine (epsilon A) and ethenocytosine (epsilon C) are formed. Although the observed mutational specificity of epsilon C suggested that it was a noninstructional lesion, the high efficiency of mutagenesis and an apparent lack of SOS dependence were reminiscent of mispairing lesions. To obtain more direct evidence showing that epsilon C has properties of a noninstructional mutagenic lesion, we have examined the in vitro template properties of a single epsilon C residue at a unique position in a synthetic oligonucleotide. The oligonucleotide was constructed by use of the following steps: (a) in vitro treatment of the pentameric oligodeoxyribonucleotide TTCTT with chloroacetaldehyde to convert the central cytosine to ethenocytosine; (b) purification and characterization of TT epsilon CTT; and (c) ligation of purified TT epsilon CTT to two decamers to create a 25 nt long oligodeoxyribonucleotide with a centrally located epsilon C residue. The template characteristics of epsilon C were examined by the annealing of end-labeled primers to the purified epsilon C-containing oligonucleotide and primer elongation by Escherichia coli DNA polymerase I in the presence of one or more nucleotide precursors. The elongation products were analyzed by high-resolution gel electrophoresis followed by autoradiography and quantitated by computing densitometry.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:1888734 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1888734 | pubmed:language | eng | lld:pubmed |
| pubmed-article:1888734 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1888734 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:1888734 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1888734 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1888734 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1888734 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1888734 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1888734 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:1888734 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:1888734 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:1888734 | pubmed:issn | 0006-2960 | lld:pubmed |
| pubmed-article:1888734 | pubmed:author | pubmed-author:HumayunM ZMZ | lld:pubmed |
| pubmed-article:1888734 | pubmed:author | pubmed-author:SimhaDD | lld:pubmed |
| pubmed-article:1888734 | pubmed:author | pubmed-author:PalejwalaV... | lld:pubmed |
| pubmed-article:1888734 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:1888734 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:1888734 | pubmed:volume | 30 | lld:pubmed |
| pubmed-article:1888734 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:1888734 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:1888734 | pubmed:pagination | 8727-35 | lld:pubmed |
| pubmed-article:1888734 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:1888734 | pubmed:meshHeading | pubmed-meshheading:1888734-... | lld:pubmed |
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| pubmed-article:1888734 | pubmed:meshHeading | pubmed-meshheading:1888734-... | lld:pubmed |
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| pubmed-article:1888734 | pubmed:meshHeading | pubmed-meshheading:1888734-... | lld:pubmed |
| pubmed-article:1888734 | pubmed:meshHeading | pubmed-meshheading:1888734-... | lld:pubmed |
| pubmed-article:1888734 | pubmed:meshHeading | pubmed-meshheading:1888734-... | lld:pubmed |
| pubmed-article:1888734 | pubmed:meshHeading | pubmed-meshheading:1888734-... | lld:pubmed |
| pubmed-article:1888734 | pubmed:meshHeading | pubmed-meshheading:1888734-... | lld:pubmed |
| pubmed-article:1888734 | pubmed:meshHeading | pubmed-meshheading:1888734-... | lld:pubmed |
| pubmed-article:1888734 | pubmed:meshHeading | pubmed-meshheading:1888734-... | lld:pubmed |
| pubmed-article:1888734 | pubmed:year | 1991 | lld:pubmed |
| pubmed-article:1888734 | pubmed:articleTitle | Mechanisms of mutagenesis by exocyclic DNA adducts. Construction and in vitro template characteristics of an oligonucleotide bearing a single site-specific ethenocytosine. | lld:pubmed |
| pubmed-article:1888734 | pubmed:affiliation | Department of Microbiology and Molecular Genetics, University of Medicine and Dentistry of New Jersey-New Jersey Medical School, Newark 07103-2714. | lld:pubmed |
| pubmed-article:1888734 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:1888734 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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