|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1991-10-4
|
|
pubmed:abstractText |
Airway inflammation is thought to play an important role in the pathogenesis of asthma. We have used in situ hybridization and an immunoassay to determine whether granulocyte macrophage colony-stimulating factor (GM-CSF) (a cytokine capable of eosinophil activation) is present in the airway of asthmatics (n = 6) who have 37.0 +/- 15.1% airway eosinophilia after endobronchial allergen challenge. Levels of immunoreactive GM-CSF (less than 4 pg/ml pre-allergen versus 180.5 +/- 46.9 pg/ml post-allergen) increased significantly 24 h after endobronchial allergen stimulation. The cellular source of bronchoalveolar lavage (BAL) GM-CSF, as determined by in situ hybridization and immunoperoxidase staining, was derived predominantly from UCHL-1 positive BAL lymphocytes, as well as from a smaller population of alveolar macrophages. Before local endobronchial allergen challenge, less than 1% of lymphocytes and alveolar macrophages recovered by BAL expressed GM-CSF mRNA, whereas after allergen stimulation 92.6 +/- 3.4% of lymphocytes and 17.5 +/- 22.7% of alveolar macrophages expressed GM-CSF mRNA. This study provides evidence that in an experimental model of allergen-induced asthma, activation of the immune and inflammatory response (BAL lymphocyte and alveolar macrophage production of GM-CSF) is temporally associated with an inflammatory cell influx of eosinophils into the airway.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885766-1176724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885766-1970229,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885766-2026869,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885766-2109776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885766-2200318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885766-2406322,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885766-2455685,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885766-2510565,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885766-2826636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885766-3095427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885766-3110347,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885766-3133397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885766-3456562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885766-3490669,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885766-3514163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885766-3680512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885766-6232459
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
AIM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0021-9738
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
88
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1048-53
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:1885766-Adolescent,
pubmed-meshheading:1885766-Adult,
pubmed-meshheading:1885766-Allergens,
pubmed-meshheading:1885766-Asthma,
pubmed-meshheading:1885766-Bronchi,
pubmed-meshheading:1885766-Bronchoalveolar Lavage Fluid,
pubmed-meshheading:1885766-Female,
pubmed-meshheading:1885766-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:1885766-Humans,
pubmed-meshheading:1885766-Macrophages,
pubmed-meshheading:1885766-Male,
pubmed-meshheading:1885766-Nucleic Acid Hybridization,
pubmed-meshheading:1885766-Pulmonary Alveoli
|
|
pubmed:year |
1991
|
|
pubmed:articleTitle |
Endobronchial allergen challenge in asthma. Demonstration of cellular source of granulocyte macrophage colony-stimulating factor by in situ hybridization.
|
|
pubmed:affiliation |
Department of Medicine, University of California, San Diego 92103.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
