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rdf:type |
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lifeskim:mentions |
umls-concept:C0014834,
umls-concept:C0017262,
umls-concept:C0044278,
umls-concept:C0080194,
umls-concept:C0185117,
umls-concept:C0204727,
umls-concept:C0205409,
umls-concept:C0679058,
umls-concept:C1265277,
umls-concept:C1519249,
umls-concept:C1547699,
umls-concept:C1868365,
umls-concept:C2700640,
umls-concept:C2911684
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pubmed:issue |
17
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pubmed:dateCreated |
1991-10-4
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pubmed:databankReference |
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pubmed:abstractText |
Pseudomonas sp. strain ACP is capable of growth on 1-aminocyclopropane-1-carboxylate (ACC) as a nitrogen source owing to induction of the enzyme ACC deaminase and the subsequent conversion of ACC to alpha-ketobutyrate and ammonia (M. Honma, Agric. Biol. Chem. 49:567-571, 1985). The complete amino acid sequence of purified ACC deaminase was determined, and the sequence information was used to clone the ACC deaminase gene from a 6-kb EcoRI fragment of Pseudomonas sp. strain ACP DNA. DNA sequence analysis of an EcoRI-PstI subclone demonstrated an open reading frame (ORF) encoding a polypeptide with a deduced amino acid sequence identical to the protein sequence determined chemically and a predicted molecular mass of 36,674 Da. The ORF also contained an additional 72 bp of upstream sequence not predicted by the amino acid sequence. Escherichia coli minicells containing the 6-kb clone expressed a major polypeptide of the size expected for ACC deaminase which was reactive with ACC deaminase antiserum. Furthermore, a lacZ fusion with the ACC deaminase ORF resulted in the expression of active enzyme in E. coli. ACC is a key intermediate in the biosynthesis of ethylene in plants, and the use of the ACC deaminase gene to manipulate this pathway is discussed.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885510-13407721,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885510-16592605,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885510-1966276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885510-2110144,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885510-2191304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885510-271968,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885510-2985470,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885510-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885510-6219389,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885510-6244898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885510-6336734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885510-6345791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885510-6350817,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885510-6423828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885510-7048259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885510-7326243,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1885510-927202
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0021-9193
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
173
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5260-5
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
pubmed-meshheading:1885510-Amino Acid Sequence,
pubmed-meshheading:1885510-Amino Acids,
pubmed-meshheading:1885510-Amino Acids, Cyclic,
pubmed-meshheading:1885510-Autoradiography,
pubmed-meshheading:1885510-Base Sequence,
pubmed-meshheading:1885510-Blotting, Western,
pubmed-meshheading:1885510-Carbon-Carbon Lyases,
pubmed-meshheading:1885510-DNA, Bacterial,
pubmed-meshheading:1885510-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:1885510-Escherichia coli,
pubmed-meshheading:1885510-Gene Expression,
pubmed-meshheading:1885510-Hydrolysis,
pubmed-meshheading:1885510-Lyases,
pubmed-meshheading:1885510-Molecular Sequence Data,
pubmed-meshheading:1885510-Open Reading Frames,
pubmed-meshheading:1885510-Pseudomonas,
pubmed-meshheading:1885510-Restriction Mapping
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pubmed:year |
1991
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pubmed:articleTitle |
Isolation, sequence, and expression in Escherichia coli of the Pseudomonas sp. strain ACP gene encoding 1-aminocyclopropane-1-carboxylate deaminase.
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pubmed:affiliation |
Calgene, Inc. Davis, California 95616.
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pubmed:publicationType |
Journal Article
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